| 摘要: |
| 克氏原螯虾亲环素蛋白A（Procambarus clarkii Cyclopllilin A，简称PcCypA）是一种存在于克氏原螯虾（P. clarkii）中高度保守的重要蛋白，具有多种生物学功能。为了初步阐明CypA在病毒侵染克氏原螯虾过程中的免疫应答机制，本研究利用qRT-PCR等技术分析了克氏原螯虾在WSSV病毒感染后，PcCypA在不同组织中的转录表达情况。研究结果表明克氏原螯虾在感染WSSV后，心脏和肌肉组织中都出现了肌纤维断裂溶解的情况，在肝胰腺组织中出现了脂肪颗粒脱落、细胞空泡化和细胞膜溶解现象。同时，不同组织中的PcCypA基因表达存在明显的时序差异，以24hpi为界，0—24hpi为注射应激期，在此期间，肝胰腺、心脏等组织中的PcCypA转录表达量均有上调；24—36hpi为应激恢复期，绝大多数的组织中PcCypA转录表达均出现了回落，但在心脏、肌肉、血淋巴组织中出现了上调，说明这三个组织对WSSV感染的响应可能早于其他组织；48hpi之后为病毒感染响应阶段，在此期间鳃和肌肉组织中PcCypA表达量显著提升，而在肝胰腺和精巢组织中却出现表达下调；96—120hpi为感染响应后期阶段，肝胰腺和肠组织的PcCypA转录水平再次上调。因而，可以初步推断PcCypA在病毒WSSV侵染克氏原螯虾过程中参与了机体的免疫应答，且在不同的组织中PcCypA的转录表达水平存在显著差异。 |
| 关键词: 克氏原螯虾 CypA qRT-PCR WSSV |
| DOI：10.11693/hyhz20180400091 |
| 分类号:Q789 |
| 基金项目:浙江省重大科技攻关项目，2014C02011号。 |
附件 |
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| EXPRESSION OF CypA AFTER WSSV CHALLENGE IN RED SWAMP CRAYFISH, PROCAMBARUS CLARKII |
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ZHU Jun-Jie1,2, LIN Feng3, WANG Yan1, ZHANG Hai-Qi3
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1.College of Animal Sciences, Zhejiang University, Hangzhou 310058, China;2.College of Life Sciences, Huzhou University, Huzhou 313000, China;3.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China
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| Abstract: |
| Cyclophilins A is a family of highly conserved and multi-functional proteins that exist extensively from microorganisms to mammals. In order to explore the immune function of CypA in Procambarus clarkii after WSSV challenge, real-time quantitative PCR was used to detect the expression characteristics after WSSV challenge. Histologic observation under a light microscope showed that the heart, the hepatopancreas and the striate muscle of the abdomen were severely affected. PcCypA transcriptional levels were significantly up-regulated in the heart and the hepatopancreas tissues at 0-24hpi. After 48hpi, CypA transcripts in the gill and the muscle were significantly increased, while transcripts in the hepatopancreas and the testicle decreased. During 96-120hpi, CypA transcripts in the hepatopancreas and the intestine were significantly increased again. The results suggested that CypA might be involved in the immune response of P. clarkii. |
| Key words: Procambarus clarkii CypA qRT-PCR WSSV |
