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应用甾醇类生物标志物追踪绿潮后期漂浮绿藻沉降区的方法
耿慧霞1,2,3, 于仁成1,2,4,3, 颜天1,2,3, 张清春1,2,3, 孔凡洲1,2,3, 周名江1
1.中国科学院海洋生态与环境科学重点实验室 青岛 266071;2.青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室 青岛 266071;3.中国科学院海洋大科学研究中心 青岛 266071;4.中国科学院大学 北京 100049
摘要:
黄海大规模绿潮的连年暴发造成了巨大的经济损失,但目前对其生态效应的认识仍非常有限。这主要是由于对绿潮后期漂浮绿藻的沉降区域缺乏了解,在很大程度上限制了对黄海绿潮生态效应的认识。本研究尝试以28-异褐藻甾醇作为绿潮藻类生物标志物,建立追踪绿藻沉降区的方法,并对其有效性和可行性进行分析和验证。研究结果表明,黄海绿潮原因种浒苔(Ulva prolifera)中主要甾醇成分为28-异褐藻甾醇,含量为50—100μg/g。在20℃和黑暗条件下,浒苔在1—2个月之内能够完全腐烂分解,腐烂分解的浒苔生物量与沉积物中保存的28-异褐藻甾醇含量呈显著正相关,沉积物中高含量的28-异褐藻甾醇能够在20℃和黑暗条件下稳定保存至少1个月,之后含量明显降低。现场调查也发现,在受绿潮影响的青岛近岸海域表层沉积物中能够检测到高含量的28-异褐藻甾醇,且其含量在绿潮消退1个月后有明显下降。因此,28-异褐藻甾醇可以作为黄海绿潮藻类的生物标志物,通过对绿潮消退后1—2月内海域表层沉积物中28-异褐藻甾醇的分析,有望揭示漂浮绿藻的沉降区域。相关结果为进一步研究黄海绿潮的生态效应奠定基础。
关键词:  黄海  绿潮  甾醇  标志物
DOI:10.11693/hyhz20180400083
分类号:Q14
基金项目:国家自然科学基金面上项目,41676106号;青岛海洋科学与技术国家实验室鳌山科技创新计划项目,2016ASKJ02号;国家自然科学基金委-山东省联合基金项目,U1606404号;中国科学院战略性先导科技专项(A类)资助,XDA11020304号。
USING STEROL BIOMARKERS TO TRACE DEPOSITION AREAS OF FLOATING GREEN ALGAE AFTER GREEN TIDES
GENG Hui-Xia1,2,3, YU Ren-Cheng1,2,4,3, YAN Tian1,2,3, ZHANG Qing-Chun1,2,3, KONG Fan-Zhou1,2,3, ZHOU Ming-Jiang1
1.CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
Outbreaks of large-scale green tides induced by green alga Ulva prolifera in the southern Yellow Sea led to huge economic losses in recent years. The ecological consequence caused by these massive green tides remains unclear due mainly to the limited knowledge on the deposition area of massive floating green algae after green tides. In this study, a method using 28-isofucosterol as a biomarker of U. prolifera to track the deposition area of floating green algae was developed. The stability and specificity of the biomarker 28-isofucosterol for bloom-forming U. prolifera were also tested. The major sterol species of the bloom-forming U. prolifera was proved to be 28-isofucosterol in the content of 50-100μg/g. In our simulation experiments in the laboratory, we found that U. prolifera could be decomposed within 1 month at 20℃ in darkness, and the 28-isofucosterol content in sediment was positively correlated with the decomposed biomass of U. prolifera. In addition, the high content of 28-isofucosterol present in the sediment remained stable within 1 month at 20℃ in darkness, then decreased rapidly. Our field investigation found a high-content of 28-isofucosterol in surface sediment collected from the coastal waters of Qingdao, which had been affected by deposited floating green algae, and the content of 28-isofucosterol dropped rapidly 1 month after the green tide. Therefore, it is believed that 28-isofucosterol can be used as a specific biomarker to trace the deposition area of floating green algae after the occurrence of green tides. The results may help further studies on the ecological consequences of green tides.
Key words:  the Yellow Sea  green tides  sterol  biomarker
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