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三疣梭子蟹(Portunus trituberculatus)雌雄性腺的差异转录组分析
汤玉洁, 棘怀飞, 王欢, 李晔
宁波大学海洋学院 宁波 315211
摘要:
三疣梭子蟹(Portunus trituberculatus)是我国东海海域具有重要经济价值的蟹类。为探讨雌雄梭子蟹性腺基因表达差异,挖掘与性腺发育相关基因,利用高通量测序技术对三疣梭子蟹雌雄性腺进行测序和生物信息学分析,为进一步研究其性腺发育及性别分化机制提供基础。测序结果经de novo组装共获得192848个转录本,130054个unigene。差异表达分析发现,其中101742个unigene上调,37588个unigene下调。在差异表达数据库中筛选出EcRRXRVTG等6个可能参与三疣梭子蟹的性腺分化发育的基因,并利用荧光定量PCR验证其在雌雄性腺中的表达情况,验证结果与转录组测序结果基本一致,为深入研究三疣梭子蟹性腺分化发育调控分子机理和相关功能基因提供了重要的基因数据资源。
关键词:  三疣梭子蟹  转录组  雌雄性腺  荧光定量PCR  基因表达
DOI:10.11693/hyhz20180500118
分类号:S917.4
基金项目:国家自然科学基金/青年科学基金项目,31402282号;浙江省重大科技专项,2012C12907-9号。
COMPARATIVE TRANSCRIPTOME ANALYSIS OF FEMALE AND MALE GONADS OF PORTUNUS TRITUBERCULATUS
TANG Yu-Jie, JI Huai-Fei, WANG Huan, LI Ye
School of Marine Science, Ningbo University, Ningbo 315211, China
Abstract:
Portunus trituberculatus is of great economic value. To investigate the mechanism of the gonadal development and sex differentiation, the different expression of genes in male and female gonads of the swimming crab were explored using transcriptome sequencing technique and bioinformatics. In the present study, 192848 transcripts and 13054 unigenes were identified through de novo assembly. Among the differential expressed genes (DEGs), 101742 were up-regulated and 37588 were down-regulated. The results of sequencing were further verified by quantitative real-time PCR (qRT-PCR). The expression of six DEGs including EcR, RXR, and VTG et al, which may be involved in gonadal development and sex determination of P. trituberculatus, were analyzed. Our results have provided clues to understand the mechanisms of gonadal development and sex differentiation in the swimming crab.
Key words:  Portunus trituberculatus  transcriptome  male and female gonad  quantitative real-time PCR  gene expression
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