| 摘要: |
| 补体系统在体液免疫中发挥着重要作用，是连接先天免疫和适应性免疫的枢纽。本研究基于前期转录组RNA-Seq数据检索马氏珠母贝（Pinctada fucata）补体样组分，并对其结构域及溶藻弧菌（Vibrio alginolyticus）刺激后的基因表达水平进行了分析。结果表明，共获得68条补体样成分unigene，分别编码14个含C1q结构域蛋白、14个凝集素蛋白、4个纤维蛋白原相关蛋白、12个丝氨酸蛋白酶、10个含硫酯键蛋白、1个末端补体分子C6、5个补体受体、1个补体因子及7个纤胶凝蛋白；结构域分析表明，马氏珠母贝补体样组分均含有相应的保守结构域，与其他贝类补体组分的研究结果基本一致；表达量分析表明，经溶藻弧菌刺激后，68条补体样组分unigene中共有21条unigene表达上调，其中12条为模式识别受体unigene。结合牡蛎等贝类补体组分的研究结果，推测贝类已形成一个含有多种补体组分的原始补体系统，且可能以凝集素途径或替代途径行使补体的生物学功能。以上研究结果为深入了解马氏珠母贝及其他海洋无脊椎动物免疫防御机制、探讨补体系统的进化起源提供了新的依据。 |
| 关键词: 马氏珠母贝 RNA-Seq 补体样组分 保守结构域 |
| DOI：10.11693/hyhz20190500095 |
| 分类号:Q789 |
| 基金项目:广东省基础与应用基础研究基金项目，2019A1515011875号；广东海洋大学2019年“冲一流”省财政专项资金建设项目，231419015号；广东省省级科技计划项目，2015A020209169号；国家级大学生创新创业训练计划项目，CXXL2018001号；广东海洋大学“海之帆-起航计划”大学生科技创新培育项目，qhjhzr201812号；广西北部湾海洋生物多样性养护重点实验室（钦州学院）开放课题，2017KB03号；国家自然科学基金项目，31202023号。 |
附件 |
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| ANALYSIS OF COMPLEMENT-LIKE COMPONENTS IN PINCTADA FUCATA BASED ON THE TRANSCRIPTOME DATASETS |
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WU Yong1, ZHANG Ye2, XU You-Hou3, LIANG Xue-Ru2, WANG Zhong-Liang2
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1.Science and Technology Department, Guangdong Ocean University, Zhanjiang 524088, China;2.Fisheries College, Guangdong Ocean University, Zhanjiang 524088, China;3.Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Beibu Gulf University, Qinzhou 535000, China
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| Abstract: |
| The complement system connecting innate immunity and adaptive immunity plays an important role in humoral immunity. In this study, the complement components of Pinctada fucata were searched based on the RNA-Seq data, and the conserved domains and expression levels after Vibrio alginolyticus challenge were analyzed. Sixty-eight complement-like component unigenes were found in RNA-Seq data, encoding 14 C1q domain-containing proteins, 14 lectins, 4 fibrinogen-related proteins, 12 serine proteases, 10 thioester-containing proteins, 1 C6, 5 complement receptors, 1 complement factor, and 7 ficolins. The complement-like components of P. fucata contained corresponding conserved domains as revealed in domain analysis, which is consistent with the results of complement components from other mollusks. The expression levels of 21 unigenes were up-regulated by V. alginolyticus challenge, of which 12 were pattern recognition receptors. Combining the results of studies on the oyster and other mollusks, we infer that a primitive complement system containing multiple components exists in P. fucata, and performs biological functions by the lectin pathway or alternative pathway. The results provide a new basis for understanding the immune defense mechanisms of P. fucata and other marine invertebrates, and for exploring the evolution of the complement system. |
| Key words: Pinctada fucata RNA-Seq complement-like component conserved domain |
