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引用本文:毛铁墙,董宏坡,陈法锦,侯庆华,朱庆梅,阳雯娜,张家伟,纪梓晗,凌炜琪.湛江湾沉积物中反硝化和厌氧氨氧化细菌的丰度、多样性及分布特征.海洋与湖沼,2020,51(1):59-74.
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湛江湾沉积物中反硝化和厌氧氨氧化细菌的丰度、多样性及分布特征
毛铁墙1, 董宏坡2, 陈法锦1, 侯庆华1, 朱庆梅1, 阳雯娜1, 张家伟1, 纪梓晗1, 凌炜琪1
1.广东海洋大学 海洋与气象学院 湛江 524088;2.华东师范大学 河口海岸学国家重点实验室 上海 200062
摘要:
采用实时荧光定量PCR、高通量测序等方法对湛江湾沉积物中四个月份的反硝化细菌与厌氧氨氧化细菌的多样性和丰度进行了分析。结果表明:湛江湾沉积物中反硝化细菌和厌氧氨氧化细菌丰度在四个月份的变化和空间分布趋势为:nirS型反硝化细菌在二月份最高,四月份最低,且其平均丰度有从湛江湾湾内向湾口附近呈现先升高再降低的趋势;nirK型反硝化细菌丰度在九月份最高,十一月份最低;nosZ型反硝化细菌在四月份最高,其余月份变化不大;厌氧氨氧化细菌丰度在九月份最高,二月份最低。通过相关性分析结果表明,亚硝酸盐、铵盐等共同调控着湛江湾沉积物中反硝化和厌氧氨氧化细菌丰度变化。系统发育分析表明:湛江湾中存在着一些广泛分布的反硝化细菌,但也生活着一些新奇的nirK型和nosZ型反硝化细菌。对于厌氧氨氧化细菌而言,其主要属于浮霉菌门及Candidatus Scalindua属,具有较高的耐盐性,另外湛江湾海区的厌氧氨氧化细菌也生活着一类在其他地方没有的新分支。典范对应分析分析结果表明:硝酸盐显著影响湛江湾反硝化细菌和厌氧氨氧化细菌的群落结构。湛江湾沉积物中反硝化细菌和厌氧氨氧化细菌存在特殊的竞争与共存的关系,且由亚硝酸盐、硝酸盐、pH等多种环境因子共同驱动。
关键词:  反硝化和厌氧氨氧化细菌  实时荧光定量PCR  高通量测序  多样性  丰度  湛江湾
DOI:10.11693/hyhz20190600108
分类号:X52
基金项目:国家自然科学基金,41576123号,41971125号;广东省自然科学基金,2018A030313164号。
ABUNDANCE, DIVERSITY, AND DISTRIBUTION OF DENITRIFIER AND ANAMMOX BACTERIA IN ZHANJIANG BAY SEDIMENTS
MAO Tie-Qiang1, DONG Hong-Po2, CHEN Fa-Jin1, HOU Qing-Hua1, ZHU Qing-Mei1, YANG Wen-Na1, ZHANG Jia-Wei1, JI Zi-Han1, LING Wei-Qi1
1.College of Ocean and Meteorology, Guangdong Ocean University, Zhanjiang 524088, China;2.State Key Laboratory of Estuarine and Coastal Research, East China Normal University, Shanghai 200062, China
Abstract:
The diversity and abundance of denitrifying bacteria and anammox bacteria in Zhanjiang Bay sediments were analyzed by real-time fluorescent quantitative PCR and high-throughput sequencing. The results show that the abundance of denitrifying bacteria and anammox bacteria in the sediments changed significantly in season and location. The nirS-type denitrifying bacteria were the highest in February and the lowest in April, and its average abundance tends to increase first and then decrease from inside to outside of the bay. The abundance of nirK-type denitrifying bacteria were the highest in September and the lowest in November. The nosZ-type denitrifying bacteria were the highest in April, and did not change in other months. The abundance of anammox bacteria were the highest in September and the lowest in February. Correlation analysis showed that nitrite and ammonium jointly regulated the changes of denitrifying and anammox bacteria abundances in the sediments. Phylogenetic analysis showed that there are some widely distributed denitrifying bacteria in Zhanjiang Bay, but certain novel nirK and nosZ-type denitrifying bacteria were also found. For anaerobic ammonium oxidizing bacteria, it mainly belongs to the Planctomycetes and Candidatus scalindua, which is high-salinity tolerated. In addition, a new branch of anammox bacteria inhabited in Zhanjiang Bay that are not found elsewhere. CCA analysis showed that nitrate significantly affected the community structure of denitrifying bacteria and anammox bacteria in the sediments. There are competition between denitrifying bacteria and anammox bacteria, which is driven by various environmental factors including nitrite, nitrate, and pH.
Key words:  denitrifier and anammox bacteria  real-time quantitative PCR  high-throughput sequencing  diversity  abundance  Zhanjiang Bay
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