引用本文:	盛鹏程,周冬仁,韩新荣,胡晓波,叶雪平,徐磊,周聃,吴琦芳,郝贵杰.不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响.海洋与湖沼,2020,51(1):148-155.

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*不同饲养方式对乌鳢(Ophiocephalus argus)肠道微生物群落结构差异及种类多样性的影响*
盛鹏程¹, 周冬仁¹, 韩新荣², 胡晓波², 叶雪平¹, 徐磊¹, 周聃¹, 吴琦芳¹, 郝贵杰¹
1.农业部淡水渔业健康养殖重点实验室浙江省鱼类健康与营养重点实验室浙江省淡水水产研究所湖州 313001;2.桐乡市水产工作站桐乡 314500

摘要:

以乌鳢（Ophiocephalus argus）为研究对象，采用不同日粮饲喂，试验W1组饲喂冰鲜鱼，试验W2组饲喂畜禽内脏。12周后，取肠道内容物，利用16S rDNA技术研究投喂不同饲粮的乌鳢肠道生物群落结构差异和微生物多样性，分别获得W1组和W2组乌鳢肠道细菌有效序列51124和39298条。结果分析表明；W1组和W2组物种注释（operational taxonomic unit，OTU）数目分别为2631和4114条，分类地位明确的细菌种类分别隶属5个属和49个属，W1组和W2组乌鳢肠道细菌种类差异很大。W1组乌鳢肠道细菌的优势类群是邻单胞菌属（Plesiomonas）、狭义梭菌属（Clostridium sensu stricto）、鲸蜡菌属（Cetobacterium）；W2组乌鳢肠道细菌的优势类群是狭义梭菌属（Clostridium sensu stricto）、邻单胞菌属（Plesiomonas）、Spartobacteria、甲基副球菌属（Methyloparacoccus）、杆菌属（Alsobacter）、Saccharibacteria、分支杆菌属（Mycobacterium）。W2组乌鳢肠道细菌香农指数高于W1组乌鳢肠道细菌，表明前者肠道细菌多样性大于后者。本实验揭示了畜禽内脏饲喂乌鳢肠道细菌的群落种类多样性、OTU丰富度和物种总数均高于冰鲜鱼饲喂乌鳢；W1组肠道有益菌OTU丰富度大于W2组，且W2组还存在一些潜在致病菌。本研究为乌鳢的健康养殖和后续肠道微生物资源的挖掘提供了理论依据。

关键词: 乌鳢(Ophiocephalus argus) Illumina MiSeq高通量测序投喂方式肠道微生物群落结构

DOI：10.11693/hyhz20190800160

分类号:S965.1;Q939.9

基金项目:浙江省科技计划项目，2016C32075号；浙江省科技计划项目，2017C02026号。

附件

EFFECTS OF DIFFERENT FEEDING MATTERS ON INTESTINAL MICROBIAL STRUCTURE DIVERSITY OF OPHIOCEPHALUS ARGUS

SHENG Peng-Cheng¹, ZHOU Dong-Ren¹, HAN Xin-Rong², HU Xiao-Bo², YE Xue-Ping¹, XU Lei¹, ZHOU Dan¹, WU Qi-Fang¹, HAO Gui-Jie¹

1.Key Laboratory of Healthy Freshwater Aquaculture, Agriculture Ministry, Key Laboratory of Fish Health and Nutrition of Zhejiang Province, Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China;2.Tongxiang Fishery Workstation, Tongxiang 314500, China

Abstract:

Snakeheaded fish Ophiocephalus argus was fed with different diets. Group W1 were fed with frozen fish and Group W2 with viscera of livestock and poultry. After 12 weeks, intestinal contents were taken and 16S rDNA technique was used to determine the difference in intestinal biodiversity and microbial diversity. Some 51 124 and 39 298 effective intestinal bacterial sequences were obtained in Groups W1 and W2, respectively. Results show that 2631 and 4114 species annotation units (OTUs) were found belonging to 5 and 49 genera in Groups W1 and W2, respectively. The intestinal bacterial species were quite different in the two groups. The dominant species in Group W1 were Plesiomonas, Clostridium sensu stricto, Cetobacterium, and Clostridium sensu stricto, while those of Group W2 were Clostridium sensu stricto, Plesiomonas, Spartobacteria, Methyloparacoccus, Alsobacter, Saccharibacteria, and Mycobacterium. The Shannon index of Group W2 was higher than that of Group W1, indicating that the diversity of intestinal bacteria in Group W2 was higher than that in Group W1. The results also show that the diversity of intestinal bacteria community, OTU richness, and total number of species fed with livestock and poultry viscera were greater than those fed with frozen fish. The OTU richness of intestinal beneficial bacteria in Group W1 was higher than that in Group W2, but Group W2 had some potential pathogens. This study provided scientific data for healthy cultivation of O. argus and potential applications of intestinal microbial resources.

Key words: snakeheaded fish Ophiocephalus argus Illumina MiSeq genome sequencing feeding methods intestinal microorganisms community structure