| 摘要: |
| 为探讨循环水养殖系统中循环率对水质因子和细菌群落多样性的影响，以黑鲷（Acanthopagrus schlegelii）为研究对象，研究了4次/天（低循环率）、8次/天（中循环率）、12次/天（高循环率）三个流量下，养殖桶和生物过滤桶两部分细菌群落结构、多样性以及细菌群落与环境因子的相关性。高通量测序结果表明：黑鲷循环水养殖中优势细菌类群为变形菌门（Proteobacteria）、拟杆菌门（Bacteroidetes）、放线菌门（Actinobacteria）、疣微菌门（Verrucomicrobia）和厚壁菌门（Firmicutes）。流量的升高可增加系统的细菌多样性，且高循环率过滤桶中具有净化水质降低营养盐功能的有益菌属更为多样化及丰度更高，同时，其系统去氨氮和总氮效果更好，去除率分别是49.5%和22.3%，氨氮去除率是低循环率组的2.9倍和中循环率的3.6倍，总氮去除率是低循环率组的8.2倍和中循环率组的20.5倍。PCoA分析和聚类分析结果表明，各实验组养殖桶与生物过滤桶中细菌群落组成有着明显的差异，中、高循环率组过滤桶菌落结构与低循环率的有明显的差异。环境因子与细菌群落结构相关性分析结果表明，总磷、活性磷酸盐、亚硝酸盐和总氮等指标对细菌群落结构影响相对较大，但未发现两者间有很显著的相关性。 |
| 关键词: 黑鲷(Acanthopagrus schlegelii) 循环水养殖 细菌群落 高通量测序 循环率 |
| DOI：10.11693/hyhz20191100209 |
| 分类号:S965 |
| 基金项目:国家自然科学基金项目，31872586号；浙江省自然科学基金项目，LY18C190008号；宁波市科技攻关项目，2017C110003号。 |
附件 |
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| EFFECTS OF FLOW RATE ON WATER QUALITY FACTORS AND MICROBIAL COMMUNITY IN RECIRCULATING AQUACULTURE SYSTEM FOR ACANTHOPAGRUS SCHLEGELII |
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LI Lu-Guo1, LUO Yun-Hui1, XU Shan-Liang1,2
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1.School of Marine Sciences, Ningbo University, Ningbo 315211, China;2.Zhejiang Key Laboratory of Marine Bioengineering, Ningbo University, Ningbo 315211, China
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| Abstract: |
| In order to investigate the effects of flow rate on water quality factors and bacterial community diversity in recirculating aquaculture systems, black cockroach (Acanthopagrus schlegelii) was taken as the research object, on which the bacterial community structure, diversity, and the correlation between bacterial communities and environmental factors of the culture barrel and biological filter barrel were studied in three flow rates of 4 (low), 8 (medium), and 12 times/d (high). High-throughput sequencing was used. Results showed that the dominant bacterial groups in the circulatory aquaculture were Proteobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia and Firmicutes. The increase of flow rate enhanced the bacterial diversity of the system. The beneficial bacteria capable of purifying water and reducing nutrient salt in high-flow filter bucket were more diverse and richer, with which the system had better effects on removing ammonia nitrogen and total nitrogen and the removal rates were 49.5% and 22.3%, respectively. The ammonia nitrogen removal rate was 2.9 times of the low rate group and 3.6 times of the medium rate group, and the total nitrogen removal rate was 8.2 times of low rate group. Double and 20.5 times of the medium rate group. Results of PCoA analysis and cluster analysis revealed significant differences in bacterial community composition between the culture barrels and the biological filter barrels. In addition, a significant difference was observed in colony structure of the biological filter barrel between the medium-high rate groups and the low rate group. Correlation analysis showed that total phosphorus, active phosphate, nitrite, and total nitrogen had relatively significant effects on bacterial community structure, but no significant correlation was found between the environmental factors and the bacterial community structure. |
| Key words: Acanthopagrus schlegelii recirculating aquaculture bacterial community high-throughput sequencing flow rate |
