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引用本文:杨伟红,郇聘,刘保忠.hedgehog基因在笠贝(Lottia goshimai)早期发育中的表达模式研究.海洋与湖沼,2020,51(4):960-965.
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hedgehog基因在笠贝(Lottia goshimai)早期发育中的表达模式研究
杨伟红1,2, 郇聘1,3,2, 刘保忠1,3,2
1.中国科学院海洋研究所 实验海洋生物学重点实验室 青岛 266071;2.中国科学院大学 北京 100049;3.青岛海洋科学与技术试点国家实验室 海洋生物学与生物技术功能实验室 青岛 266237
摘要:
Hedgehog信号通路参与动物神经系统发育、分节等诸多发育过程,研究该信号通路对理解动物的发育与演化机制有重要意义。对软体动物而言,目前Hedgehog信号通路的研究较少。本文克隆了腹足纲软体动物笠贝的hedgehog基因,利用整装原位杂交技术研究了其在胚胎发育过程中的时空表达规律;并与重要发育基因brachyurysoxb的表达模式进行了比较。结果表明,笠贝hedgehog基因与神经外胚层及中/内胚层相关,可能调控了幼虫神经、肌肉及消化道的分化。通过比较原肠胚及担轮幼虫不同阶段的基因表达模式,研究了hedgehog基因相关的组织和细胞随个体发育进程的动态变化。这些结果为理解软体动物形态发生和重要器官的分化机制提供了基础。
关键词:  笠贝  hedgehog基因  发育  神经系统
DOI:10.11693/hyhz20200100005
分类号:Q344
基金项目:山东省重大科技创新工程专项课题,2018SDKJ0302-1号。
EXPRESSION PATTERNS OF HEDGEHOG GENE DURING EARLY DEVELOPMENT OF THE MOLLUSK LOTTIA GOSHIMAI
YANG Wei-Hong1,2, HUAN Pin1,3,2, LIU Bao-Zhong1,3,2
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
Abstract:
Hedgehog signaling pathway is involved in many aspects of animal development, such as neurogenesis and segmentation. It has been evidenced that hedgehog signaling regulates many conserved developmental events in bilaterians, which is essential to understand the development and evolution of animals. However, for mollusks, the roles of Hedgehog signaling pathway remain largely unknown. Therefore, we identified hedgehog gene from a gastropod Lottia goshimai and investigated its temporal-spatial expression patterns during development using whole mount in-situ hybridization. The hedgehog expression was compared with those of other important developmental genes brachyury and soxb, which exhibited the structure of the neuroectoderm in L. goshimai embryo. Our results showed that hedgehog gene was associated with the neuroectoderm and meso/endodermal tissues, indicating that the gene might contribute to the development of nervous system, muscle, and the digestive tract. These results provide fundamental supports for understanding the mechanisms of morphogenesis and the differentiation of important organs in mollusks.
Key words:  Lottia goshimai  hedgehog gene  development  nervous system
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