| 摘要: |
| 近几年，紫菜“高温烂菜”现象频发，为开发耐高温紫菜栽培种质，本文研究了4种温度（15、20、25、28℃）对皱紫菜壳孢子萌发及叶状体形态建成的影响，并观察了藻体的繁殖特性。结果显示，在试验设定的培养条件下，适宜皱紫菜壳孢子萌发生长温度为20—25℃，最适温度为20℃。皱紫菜壳孢子幼苗萌发初期细胞分裂为横分裂，叶状体细胞呈直线排列，生长至7—10个细胞后，细胞开始出现纵分裂。15、20℃温度组藻体多为不规则的半圆形裂片。25、28℃温度组藻体多为细长型叶片。有性生殖是皱紫菜主要的繁殖方式，但15—20℃培养组的部分藻体能够少量形成并放散类似无性生殖的单孢子并萌发形成叶状体。 |
| 关键词: 皱紫菜 壳孢子 萌发 形态建成 |
| DOI：10.11693/hyhz20191200256 |
| 分类号:Q943.1;S917 |
| 基金项目:国家重点研发计划“蓝色粮仓科技创新”专项项目，2018YFD0901502-3号，2018YFD0901506-3号；国家藻类体系，CARS-50号；江苏现代农业（紫菜）产业技术体系，JATS[2019]393号，JATS[2019]394号，JATS[2019]395号；江苏省农业科技自主创新资金项目，CX（19）3013号，CX（19）3017号；南通市科技计划项目，MS12018085号，YYZ17001号；江苏省海洋水产研究所青年科学基金，SQ201702号 |
附件 |
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| EFFECT OF TEMPERATURE ON CONCHOSPORE GERMINATION AND BLADES MORPHOGENESIS OF PYROPIA CRISPATA (BANGIALES, RHODOPHYTA) |
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TIAN Cui-Cui1,2, LU Qin-Qin1, ZHU Jian-Yi3, DENG Yin-Yin1, HU Chuan-Ming1, XU Guang-Ping1, ZHOU Wei1,2
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1.Jiangsu Marine Fisheries Research Institute, Nantong 226007, China;2.Protection and Utilization of Agricultural Germplasm Resources in Jiangsu Province, Nanjing 210014, China;3.Department of Biology and Food Engineering, Changshu Institute of Technology, Changshu 215500, China
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| Abstract: |
| In recent years, cases of Pyropia thallus decay due to high-temperature stress are reported frequently. To develop high-temperature resistant strains of Pyropia, we studied the effects of temperatures (15, 20, 25, and 28℃) on the conchospore germination and blades morphogenesis of P. crispata, and observed the reproduction characteristics. Under the culture conditions in the experiment, the suitable temperature range for the conchospore germination of P. crispata was 20—25℃, and the optimum temperature was 20℃. In the early development stage of P. crispata seedlings, the cell division was transverse, resulting in the in-line arrange of the blades cells. After growing up to 7—10 cells, the cells began to divide vertically. At 15℃ and 20℃, the most blades of P. crispate showed irregular semicircular. At 25℃ and 28℃, the blades were easier to grow in a linear way. Sexual reproduction was the main mode of P. crispate. However, some of the thallus could form and release archeospore-like cells and germinate into thallus at 15—20℃. |
| Key words: Pyropia crispata conchospore germination morphogenesis |
