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引用本文:董甜甜,崔彦婷,王忠凯,龙劲男,陈怡更,王聪,赵楠楠,王仁杰,李玉全.高盐胁迫对凡纳滨对虾(Litopenaeus vannamei)生长、代谢和抗氧化酶活力的影响.海洋与湖沼,2020,51(5):1239-1245.
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高盐胁迫对凡纳滨对虾(Litopenaeus vannamei)生长、代谢和抗氧化酶活力的影响
董甜甜, 崔彦婷, 王忠凯, 龙劲男, 陈怡更, 王聪, 赵楠楠, 王仁杰, 李玉全
青岛农业大学海洋科学与工程学院 青岛 266109
摘要:
为分析高盐胁迫对凡纳滨对虾(Litopenaeus vannamei)生长、代谢和抗氧化酶活力的影响,实验以平均体重(3.0±0.4)g,平均体长(7.6±0.2)cm的凡纳滨对虾为实验材料,设置25(对照组)、35、45、55四个盐度梯度,分别于实验开始后0d、10d、20d、30d取样,检测对虾体重、体长、甘油三酯(TG)、总胆固醇(TC)和三磷酸腺苷(ATP)的含量以及苹果酸脱氢酶(MDH)、总超氧化物歧化酶(T-SOD)、过氧化氢酶(CAT)活力,计算增重率、增长率、特定生长率和成活率。结果表明,随着盐度升高,凡纳滨对虾的增重率、增长率、特定生长率和存活率均逐渐降低,55盐度组各生长指标均显著低于对照组(P<0.05);10d时TG含量随着盐度的增加逐渐降低,55盐度组显著低于对照组(P<0.05)。20d和30d时逐渐升高,55盐度组显著高于对照组(P<0.05);55盐度组的TC含量最高,且30d时显著高于其他组(P<0.05);45盐度组、55盐度组MDH活力在10d时显著低于对照组(P<0.05),但20d和30d时显著高于对照组(P<0.05);35盐度组、45盐度组ATP含量在10d和20d时升高,且显著高于对照组(P<0.05);各处理组T-SOD和CAT的活力整体高于对照组,且随着实验时间的延长,呈上升趋势。研究结果表明,高盐胁迫不利于凡纳滨对虾的生长和存活,但可以激活凡纳滨对虾的抗氧化能力,使抗氧化酶活力升高,并显著影响对虾的脂类代谢。
关键词:  凡纳滨对虾  高盐  生长  代谢  抗氧化
DOI:10.11693/hyhz20200300082
分类号:S968.22;Q955
基金项目:山东省现代农业产业技术体系虾蟹类创新团队,SDAIT-15-011号;山东省海水养殖病害防治重点实验室开放基金,KF201901号;山东省园区产业提升工程,2019YQ003号;山东省重点研发计划-鲁渝科技协作项目,2019LYXZ008号&cstc2019jscx-lyjsAX0003号;山东省海洋与渔业科技创新计划,2017YY20号
EFFECT OF HIGH-SALINITY STREE ON GROWTH, METABOLISM, AND ANTIOXIDANT ENZYME ACTIVITIES OF LITOPENAEUS VANNAMEI
DONG Tian-Tian, CUI Yan-Ting, WANG Zhong-Kai, LONG Jin-Nan, CHEN Yi-Geng, WANG Cong, ZHAO Nan-Nan, WANG Ren-Jie, LI Yu-Quan
Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China
Abstract:
To analyze the effect of high-salinity stress on the growth, metabolism, and antioxidant enzyme activities of Litopenaeus vannamei, the shrimps in average body weight (3.0±0.4)g and average body length (7.6±0.2)cm were used as the experimental materials. Four salinity gradients of 25 (control group), 35, 45, and 55 were set up. Samples were taken at on Days 0, 10, 20, and 30. The body weight, body length, the contents of triglyceride (TG), total cholesterol (TC), and adenosine triphosphate (ATP), the activities of malate dehydrogenase (MDH), total superoxide dismutase (T-SOD), and catalase (CAT) were measured, and weight gain rate, body length growth rate, specific growth rate, and survival rate were calculated. The results show that body length growth rate, weight gain rate, specific growth rate, and survival rate of L. vannamei decreased gradually with the increase of salinity. The growth indexes of salinity 55 group were significantly lower than that of the control group (P<0.05). The TG content of salinity 55 group decreased gradually with the increase of salinity on Day 10, and was significantly lower than that of the control group (P<0.05). The TC content of salinity 55 group was higher than that of the control group (P<0.05). The MDH activities of salinity 45 and 55 groups were significantly lower than that of the control group on Day 10 (P<0.05), but significantly higher than that of the control group on Days 20 and 30 (P<0.05). ATP contents of salinity 35 and 45 groups increased on Days 10 and 20, and were significantly higher than that of the control group (P<0.05), but ATP contents of all treatment groups were decreased on Day 30. The activities of T-SOD and CAT in treatment groups were higher than the control groups, and the activities showed an upward trend with the increase of experimental time. The results show that high-salinity stress was not conducive to the growth and survival of L. vannamei, but it could activate the antioxidant activity by increasing the activity of antioxidant enzymes, and significantly affect the lipid metabolism of L. vannamei.
Key words:  Litopenaeus vannamei  high-salinity  growth  metabolism  antioxidant
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