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引用本文:王再明,王健鑫,苑文凤,李鹏.兼性厌氧海洋细菌Shewanella sp.N3B_R的分离鉴定及产电性能分析研究.海洋与湖沼,2021,52(1):175-185.
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兼性厌氧海洋细菌Shewanella sp.N3B_R的分离鉴定及产电性能分析研究
王再明, 王健鑫, 苑文凤, 李鹏
浙江海洋大学海洋科学与技术学院 舟山 316022
摘要:
本研究的目的是利用聚苯胺修饰双室微生物燃料电池(MFC),从海水养殖废水中分离筛选阳极海洋产电菌,并利用响应面Box-Behnken设计优化各产电因子,提高产电性能。从海水养殖池塘的废水和污泥中分离出一株兼性厌氧海洋菌株,利用16S rRNA基因序列和API 20NE生化分析进行鉴定,采用扫描电镜进行菌株形态的观察。采用循环伏安法(CV)和电化学阻抗谱法(EIS)研究了不同阳极电极在MFC中的性能。采用Box-Behnken设计(BBD)对复合模式进行优化。结果显示:分离到的阳极海洋产电菌株鉴定为希瓦氏菌Shewanella sp.N3B_R,聚苯胺修饰阳极可以减少MFC的电荷转移电阻,输出电压和功率密度比未经修改的碳布电极分别提高了3倍和10倍。BBD结果表明,最适温度为35℃,NaCl和苯胺浓度分别为5 g/L和0.15 mol/L,得到的功率密度最高产率为522.1 mW/m2
关键词:  兼性厌氧海洋细菌  微生物燃料电池(MFC)  16S rRNA  Box-Behnken  希瓦氏菌(Shewanella sp.)
DOI:10.11693/hyhz20200500143
分类号:O646;X172
基金项目:国家重点研发项目“蓝色粮仓科技创新”重点专项,2019YFD0901305号;舟山市科技局项目,2019C21011号;舟山市科技局项目,2016C41001号;2019年福建省海洋生物增养殖与高值化利用重点实验室开放课题基金项目,2019fjscq04号。
ISOLATION AND IDENTIFICATION OF FACULTATIVE ANAEROBIC MARINE BACTERIUM SHEWANELLA SP. N3B_R AND ANALYSIS OF ITS ELECTRICAL PERFORMANCE IN A TWO-COMPARTMENT MICROBIAL FUEL CELL
WANG Zai-Ming, WANG Jian-Xin, YUAN Wen-Feng, LI Peng
School of Ocean Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
Abstract:
A strain was isolated from wastewater and sludge in mariculture ponds and identified using 16S rRNA gene sequences and API 20NE biochemical analysis. The isolated strain was identified as Shewanella sp. N3B_R and was used as a biocatalyst on an anode in a dual-chamber microbial fuel cell (MFC), and the electrochemical performance was investigated. Electrochemical impedance spectroscopy (EIS) analysis revealed that modifying an anode with polyaniline could reduce the charge transfer resistance in the MFC, resulting in 3 and 10 times higher output voltage and power density, respectively, than the corresponding MFC with an unmodified carbon cloth electrode. Optimization was achieved using Box-Behnken design of response surface method, from which the optimum conditions were:temperature of 35℃, 5 g/L of aniline, and 0.2 mol/L of NaCl in concentration, under which the maximum yield of power density obtained was 522.1 mW/m2.
Key words:  facultative  microbial fuel cell (MFC)  16S rRNA  Box-Behnken  Shewanella sp.
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