| 摘要: |
| 微管是细胞骨架的主要成分，其结构及动力学机制对提高生物体耐受性具有重要作用。微管网络如何通过结构的动态变化调控适应环境变化已成为胁迫生物学领域的研究热点之一。条斑紫菜（Pyropia yezoensis）能够适应潮间带复杂多变的环境，是研究潮间带大型海藻抗逆机制的良好材料。目前对紫菜微管相关基因家族构成及其生物学功能的研究较少。本研究利用生物信息学方法，在条斑紫菜基因组中共鉴定出4个Tubulin基因（Pyα-tubulin 1、Pyα-tubulin 2、Pyβ-tubulin和Pyγ-tubulin）和11个Kinesin基因（PyKinesin 1—PyKinesin 11），并对其理化性质、基因结构、蛋白特征、染色体定位、系统进化和失水胁迫下的表达模式进行了系统分析。结果显示：条斑紫菜中有3种微管蛋白（Tubulin）亚型；该家族成员散布于1号和2号染色体上，Pyα-tubulin 1和Pyα-tubulin 2为串联重复基因；PyTubulin家族成员在基因结构和蛋白特征方面均较为保守，且在转录水平对失水胁迫不敏感。条斑紫菜中有5种驱动蛋白（Kinesin）亚型，亚家族种类和基因数量均少于高等植物；该家族基因散布于1号、2号和3号染色体上，无串联重复基因；PyKinesin家族成员在基因结构和蛋白特征方面存在一定差异，PyKinesin 1在中高度失水胁迫下表达量显著上调。本研究为进一步理解Tubulin和Kinesin基因家族的进化和功能、解析微管在条斑紫菜响应失水胁迫中的作用奠定了基础。 |
| 关键词: 条斑紫菜 Tubulin Kinesin 基因家族 失水胁迫 |
| DOI：10.11693/hyhz20200600174 |
| 分类号:S917.3 |
| 基金项目:国家自然科学基金项目，41976146号，31672641号。 |
附件 |
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| IDENTIFICATION AND EXPRESSION PATTERN ANALYSIS OF TUBULIN AND KINESIN GENE FAMILY IN PYROPIA YEZOENSIS TO DEHYDRATION STRESSES |
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CUI Zheng-Cai1, KONG Fan-Na1, MAO Yun-Xiang1,2, SUN Bin1, WANG Jun-Hao1, DONG Dao-Ying1
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1.The Key Laboratory of Marine Genetics and Breeding, Ministry of Education, Ocean University of China, Qingdao 266003, China;2.Key Laboratory of Utilization and Conservation of Tropical Marine Bioresource (Ministry of Education), College of Fisheries and Life Science, Hainan Tropical Ocean University, Sanya 572022, China
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| Abstract: |
| Microtubules, the main components of the cytoskeleton, play vital important roles in improving the tolerance of organisms by altering their structures in dynamic mechanisms; and they have become one of the research hotspots in the field of stress biology. Pyropia yezoensis is an ideal candidate macroalgae for the investigation of the mechanism of stress resistance because of its fast-changing living conditions in intertidal zone. At present, few studies reported the compositions and biological functions of microtubule-related gene families of P. yezoensis. In this study, we identified four Tubulin genes (Pyα-tubulin 1, Pyα-tubulin 2, Pyβ-tubulin, and Pyγ-tubulin) and 11 Kinesin genes (PyKinesin 1-PyKinesin 11) in P. yezoensis genome using bioinformatics methods. The physicochemical properties, gene structures, protein characteristics, chromosomal locations, phylogenetic evolution, and expression patterns of the two gene families in P. yezoensis under dehydration stress were systematically analyzed. The results show that four Tubulin genes could be divided into three subclusters of Tubulin gene families and they were scattered on chromosome 1 and chromosome 2 of P. yezoensis. Pyα-tubulin 1 and Pyα-tubulin 2 were tandem repeat genes. The PyTubulin family was conserved in gene and protein structures and insensitive to dehydration stress in transcription level. Five subclusters of Kinesin gene families were identified among the 11 Kinesin genes in P. yezoensis, which was less than that of higher plants. PyKinesins were scattered on all the three chromosomes of P. yezoensis and there were no tandem repeat genes. The PyKinesin families had certain differences in gene and protein structures. The expression of PyKinesin 1 was significantly enhanced under the medium and high dehydration stress. This study shall laid foundation for further analysis of microtubules in respond to dehydration stress of P. yezoensis. |
| Key words: Pyropia yezoensis Tubulin Kinesin gene family dehydration stress |
