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引用本文:何渊,杨娟娟,沈颂东.浒苔光照和盐度胁迫响应基因UpMYB44的克隆与表达分析.海洋与湖沼,2021,52(5):1191-1200.
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浒苔光照和盐度胁迫响应基因UpMYB44的克隆与表达分析
何渊, 杨娟娟, 沈颂东
苏州大学基础医学与生物科学学院 苏州 215000
摘要:
为证明R2R3-MYB转录因子在浒苔响应非生物胁迫例如盐度和光照的过程中发挥的重要调控作用,利用实时荧光定量PCR、酵母双杂交系统、亚细胞定位等技术,研究获得了浒苔UpMYB44基因1 437 bp的开放阅读框(ORF)全长序列,编码478个氨基酸,属于典型的R2R3-MYB转录因子并通过烟草叶片转化确定其定位于细胞核,UpMYB44参与浒苔响应光照和盐度的胁迫过程,其中在低光和高盐胁迫下UpMYB44基因的相对表达量升高。筛选出了与UpMYB44互作的UpCPP5蛋白,该蛋白与浒苔的生长和细胞分裂有关,推测UpMYB44可能通过与UpCPP5互作,形成蛋白复合体参与浒苔的增殖过程。研究为日后深入探究MYB类转录因子家族调控藻类生长发育的过程奠定了基础,同时为研究浒苔快速繁殖的机制提供了思路。
关键词:  浒苔  UpMYB44  转录因子  光照  盐度  快速繁殖
DOI:10.11693/hyhz20210200038
分类号:Q943.2
基金项目:国家重点研发计划资助项目,2016YFC1402102号;自然资源部海洋生态环境科学与技术重点实验室开放基金资助项目,MEEST-2020-2号;中国博士后科学基金资助项目,2020M681698号;江苏省自然科学基金资助项目,BK20200882号;江苏省博士后科研资助计划,2020Z300号;江苏省高等学校自然科学研究项目,20KJD170004号。
附件
CLONING AND EXPRESSION OF THE UPMYB44 GENE IN RESPONSE TO LIGHT AND SALINITY STRESS IN ULVA PROLIFERA
HE Yuan, YANG Juan-Juan, SHEN Song-Dong
College of Biology and Basic Medical Sciences, Soochow University, Suzhou 215000, China
Abstract:
To prove that the R2R3-MYB transcription factor plays an important regulatory role in the process of Ulva prolifera responding to abiotic stresses such as salinity and light, quantitative real-time PCR, yeast two-hybrid system, subcellular localization, and other technologies were used. A 1 437 bp full-length sequence of open reading frame (ORF) of UpMYB44 in Ulva prolifera was obtained, encoding 478 amino acids. The amino acid sequence was analyzed using bioinformatics methods. Results show that there was a typical R2R3-MYB transcription factor and it was found located in the nucleus trough transformation of Tobacco leaf. The UpMYB44 gene responded to the low light and high salinity stresses of U. prolifera by increasing its relative expression. Meanwhile, the UpCPP5 protein interactive with UpMYB44 was found, and this protein was related to the growth and cell division of U. prolifera. We speculated that UpMYB44 might interact with UpCPP5 and form a protein complex with which the proliferation of U. prolifera was promoted. This study enhanced our understanding of the MYB transcription factors in regulation, growth, and development of the algae, and provided an insightful idea for studying the rapid growth mechanism of U. prolifera.
Key words:  Ulva prolifera  UpMYB44  transcription factor  light  salinity  rapid growth
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