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引用本文:杨佳丽,冯泽中,牛建峰,顾文辉,何帮翔,刘雪华,邵之卓,郑阵兵,王旭雷,王广策.低盐、高温诱导的条斑紫菜(Pyropia yezoensis)病烂机制研究.海洋与湖沼,2021,52(5):1214-1223.
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低盐、高温诱导的条斑紫菜(Pyropia yezoensis)病烂机制研究
杨佳丽1,2, 冯泽中1,3, 牛建峰1,4,5, 顾文辉1,4,5, 何帮翔1,2, 刘雪华1,2, 邵之卓1,2, 郑阵兵1,4,5, 王旭雷1,4,5, 王广策1,4,5
1.中国科学院海洋研究所实验海洋生物学重点实验室 青岛 266071;2.中国科学院大学 北京 100049;3.青岛农业大学海洋科学与工程学院 青岛 266109;4.青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 青岛 266237;5.中国科学院海洋大科学研究中心 青岛 266071
摘要:
条斑紫菜(Pyropia yezoensis)是中国北方沿海大面积栽培的一种重要大型经济海藻。近些年,因高温等环境因子导致的烂菜时有发生,严重制约了产业的可持续发展。测定分析了不同温度(15℃和20℃)、不同盐度(20,25和30)条件对条斑紫菜叶状体光合作用参数、抗氧化酶系统活性及相关分子表达量变化的影响。发现高温(20℃)和低盐(盐度20)胁迫下会导致潜在最大量子产额(Fv/Fm)下降、非光化学淬灭Y(NO)值上升;同时,高温低盐(20℃,盐度20)条件下丙二醛(MDA)含量显著提高,表明条斑紫菜叶状体光合作用系统已受到一定的损伤。另外,过氧化氢酶(CAT)与抗坏血酸过氧化物酶(APX)活性随盐度降低而下降,在盐度20和高温(20℃)时其酶活显著降低,而超氧化物歧化酶(SOD)和过氧化物酶(POD)活性显著上升。而荧光定量结果显示,在20℃高温条件下,SOD转录出现轻微上调。随着盐度的降低,POD转录水平显著下调。此外,20℃高温胁迫下,低盐盐度20条件下,相关抗氧化酶系统关键基因的表达均出现显著下调,表明在高温、低盐胁迫下,藻体内氧自由基清除能力受到抑制,最终导致细胞受到氧化损伤。因此,本实验表明,在高温条件下、盐度降低导致的抗氧化系统酶活及相关基因表达受到抑制,这是紫菜病烂发生的诱因之一。
关键词:  活性氧清除系统  高温胁迫  光合作用  逆境响应机制  条斑紫菜
DOI:10.11693/hyhz20210300057
分类号:Q539;Q51
基金项目:山东省重点研发计划重大科技创新工程项目,2019JZZY010815号;国家自然科学基金项目,41776150号;财政部和农业农村部:国家现代农业产业技术体资助。
附件
PRELIMINARY STUDY ON THE MECHANISM OF ROTTEN DISEASES INDUCED BY LOW SALINITY AND HIGH TEMPERATURE IN PYROPIA YEZOENSIS
YANG Jia-Li1,2, FENG Ze-Zhong1,3, NIU Jian-Feng1,4,5, GU Wen-Hui1,4,5, HE Bang-Xiang1,2, LIU Xue-Hua1,2, SHAO Zhi-Zhuo1,2, ZHENG Zhen-Bing1,4,5, WANG Xu-Lei1,4,5, WANG Guang-Ce1,4,5
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China;4.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;5.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
Pyropia yezoensis is an important economic macroalgae cultivated in the coastal areas of northern China. In recent years, increased temperature and other environmental factors caused rotten diseases frequently, which restricted seriously the sustainable development of the aquaculture of P. yezoensis. We studied the effects of temperature (15℃ and 20℃) and salinity (20, 25, and 30) on photosynthetic parameters, activity and transcription of antioxidant enzymes of P. yezoensis thallus. Results show that condition of 20℃ and salinity 20 resulted in the decrease of potential maximum quantum yield (Fv/Fm) and the increase of quantum yield of nonregulated energy dissipation Y(NO). Meanwhile, under the condition, the photosynthetic system of P. yezoensis was seriously affected as indicated by obviously increased content of malondialdehyde (MDA). On the other hand, with the decrease in the salinity, the activities of catalase (CAT) and ascorbate peroxidase (APX) decreased significantly, while the activities of superoxide dismutase (SOD) and peroxidase (POD) increased dramatically but increased slightly when salinity decreased, indicating that salinity effect was small. SOD transcription level was up-regulated slightly at 20℃, while POD transcription level was down-regulated significantly with decrease of the salinity. In addition, under high temperature and low salinity stress condition (20℃, salinity 20), the expression of key genes of antioxidant enzyme system was significantly down-regulated under high temperature and low salinity stress (20℃, 20), and the scavenging of oxygen radical species was inhibited, which eventually led to the oxidative damage of cells. Therefore, under the conditions of high temperature and low salinity, the inhibition of antioxidase activity and related gene expression might explain the reason in biomolecular of the rotten diseases in aquaculture of P. yezoensis.
Key words:  scavenging system of ROS  high temperature stress  photosynthesis  stress response mechanism  Pyropia yezoensis
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