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引用本文:徐小杰,王学颖,周莉,刘清华,李军.许氏平鲉(Sebastes schlegelii)卵子发生及妊娠过程中雌激素、芳香化酶及其受体的变化规律.海洋与湖沼,2021,52(5):1236-1243.
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许氏平鲉(Sebastes schlegelii)卵子发生及妊娠过程中雌激素、芳香化酶及其受体的变化规律
徐小杰1,2,3,4, 王学颖1,2,3, 周莉1,2,3,4, 刘清华1,2,3, 李军1,2,3
1.中国科学院海洋研究所实验海洋生物学重点实验室 青岛 266071;2.中国科学院海洋大科学研究中心 青岛 266071;3.青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 青岛 266237;4.中国科学院大学 北京 100049
摘要:
雌二醇是鱼类卵巢分泌的主要的性类固醇激素之一,在脊椎动物卵子发生、性别分化、卵黄形成、生理免疫以及中枢神经系统调节等生理过程中发挥重要的作用。许氏平鲉营胎生繁殖,且经过漫长的卵黄积累期和妊娠期,这一特殊的生殖方式使得它受到了国内外学者的广泛关注。通过分析与雌二醇合成及作用密切相关的cyp19aEra在许氏平鲉卵巢上的定位、cyp19acyp19b mRNA在不同卵巢发育时期及不同组织中的表达以及不同发育期的卵巢中雌二醇含量变化,发现在卵巢发育的过程中,许氏平鲉体内雌二醇含量的峰值出现在IV期和体节期。并且cyp19acyp19b在卵巢中均有表达且在不同的卵巢发育期表达量不同。在卵黄积累的过程中,cyp19acyp19b在IV期的表达量最高,在妊娠时体节期表达量最高。不仅如此,在V期卵巢的滤泡层、基质和囊胚期的滤泡胎盘上检测到了强烈的Era的信号。这些结果说明雌二醇在胎生鱼类卵子发生及妊娠过程中均起到非常重要作用。这一研究为深入解析许氏平鲉胎生生殖特性积累了资料。
关键词:  许氏平鲉  滤泡胎盘  雌激素  雌激素受体  芳香化酶
DOI:10.11693/hyhz20210200054
分类号:Q492;S917
基金项目:国家重点研发计划,2018YFD090125号,2018YFD090124号;山东省重点基金,ZR2020KC038号;财政部和农业农村部:国家现代农业产业技术体系资助,CARS-47号;山东省农业重大应用技术创新项目,SD2019YY011号;山东省重大科技创新计划,2019JZZY020710号;国家海洋水产种质资源库项目资助。
附件
CHANGES OF ESTROGEN, AROMATASE AND ESTROGEN RECEPTOR DURING OOGENESIS AND GESTATION OF SEBASTES SCHLEGELII
XU Xiao-Jie1,2,3,4, WANG Xue-Ying1,2,3, ZHOU Li1,2,3,4, LIU Qing-Hua1,2,3, LI Jun1,2,3
1.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;3.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
Estradiol is one of the main sex steroid hormones secreted from fish ovaries. It plays an important role in the physiological processes of vertebrate oogenesis, sex differentiation, yolk formation, physiological immunity, and central nervous system regulation. Sebastes schlegelii is a viviparous fish, and has a long period of yolk accumulation and pregnancy. This special reproductive mode has drawn attention from international scholars. The location of cyp19a and Era, which are closely related to the synthesis and role of estrogen on the ovariy of black rockfish, the expression of cyp19a and cyp19a1b mRNA in different ovarian development stages and different tissues, and the level of estradiol in ovaries at different development stages were analyzed. Results show that in the process of ovarian development, the peak of estradiol content in black rockfish appeared in stage Ⅳ and somite. In addition, both cyp19a and cyp19b were expressed in the ovary and the expression levels were different in different ovarian development stages. cyp19a and cyp19b showed the highest expression levels at stage Ⅳ during oogenesis, and somatic period during gestation. In addition, strong Era signals were detected on the follicular layer, stroma at stage V of the ovary and the follicular placenta at blastocyst stage. These results provide a new interpretation to the ovarian development and viviparous reproductive strategy of black rockfish.
Key words:  black rockfish Sebastes schlegelii  follicular placenta  estrogen  estrogen receptor  P450aromatase
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