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引用本文:董正,曾启繁,刘亮洁,王师.栉孔扇贝Smad基因家族的表达特征和对鳗弧菌侵染的免疫应答分析.海洋与湖沼,2021,52(5):1273-1283.
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栉孔扇贝Smad基因家族的表达特征和对鳗弧菌侵染的免疫应答分析
董正1, 曾启繁1,2, 刘亮洁1, 王师1,2
1.中国海洋大学海洋生命学院 海洋生物遗传学与育种教育部重点实验室 青岛 266003;2.青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 青岛 266237
摘要:
栉孔扇贝是我国海水养殖业的重要品种之一,但养殖扇贝陆续暴发大规模细菌性疾病,造成了巨大的经济损失。深入研究扇贝的免疫防御机制,是改良种质、培育抗病品系的必要措施。近年来研究发现,Smad基因可以通过调控增强子结合蛋白(CEBP/ε)来促进中性粒细胞的成熟和功能极化,在引发脊椎动物的先天免疫调节中发挥着重要的作用。然而目前对于Smad及其在免疫中的作用的研究大都集中于脊椎动物中,在海洋贝类中的研究还较为匮乏。因此,对栉孔扇贝的Smad基因家族进行了系统发生、共表达网络的构建、以及鳗弧菌侵染的免疫应答分析,以解析贝类Smad基因家族的表达调控机制和在免疫应答中的作用。结果表明,Smad3Smad6可能在两侧对称动物的共同祖先中位于同一染色体上;栉孔扇贝Smad5Toll样受体信号通路的关键基因协同表达;受鳗弧菌侵染后,血淋巴中的Smad5显著上调,说明Smad5基因可能通过与Toll样受体信号通路相互作用,参与了机体的免疫应答。研究结果可为深入研究贝类Smad基因在天然免疫系统中的作用提供重要参考。
关键词:  栉孔扇贝  Smad基因家族  表达模式  免疫应答反应
DOI:10.11693/hyhz20210200029
分类号:G173
基金项目:国家自然科学基金青年科学基金项目,32002388号。
附件
EXPRESSION CHARACTERISTICS OF Smad GENE FAMILY AND ITS IMMUNE RESPONSE TO VIBRIO ANGUILLARUM INFECTION IN CHLAMYS FARRERI
DONG Zheng1, ZENG Qi-Fan1,2, LIU Liang-Jie1, WANG Shi1,2
1.Key Laboratory of Marine Genetics and Breeding(Ministry of Education), College of Marine Life Science, Ocean University of China, Qingdao 266003, China;2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China
Abstract:
Chlamys farreri is one of the most important scallops in Chinese mariculture industry. However, bacteria-caused diseases occasionally break out in scallop aquaculture in recent years, resulting in huge economic losses. Therefore, the immune defense mechanism of scallop was studied to improve germplasm and cultivate disease resistant strains. It has been found that Smad genes could mediate neutrophil maturation and functional polarization by regulating enhancer binding protein ε (cebp/ε), which plays an important role in the innate immune regulation of vertebrates. At present, most of the researches on Smad genes and their roles in immunity focused on vertebrates, those on shellfish are relatively scarce. Therefore, we characterized the phylogeny of Smad gene family in C. farreri, constructed the co-expression network, and analyzed the immune response to V. anguillarum infection, in order to analyze the expressional regulation mechanism of Smad genes in shellfish and its role in immune response. The results show that Smad3 and Smad6 co-chromosomes existed in the common ancestor of bilateran animals. Smad5 was co-expressed with a large number of key genes of Toll-like receptor signaling pathway in C. farreri. Smad5 was significantly up-regulated in hemolymph after infection by V. anguillarum, which indicated that Smad5 might participate in the immune function through interaction with Toll like receptor signaling pathway. This study provides an important reference for further study on the role of Smad genes in the innate immune system of shellfish.
Key words:  Chlamys farreri  Smad gene family  expression pattern  immune responses
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