| 摘要: |
| 硬骨鱼类具有独特的免疫系统，其头肾和体肾是重要的造血和免疫器官。为探索黄颡鱼头肾和体肾造血和免疫功能异同，采用Illumina HiSeq NovaSeq 6000测序平台对黄颡鱼头肾和体肾进行了转录组高通量测序；测序序列经质控、组装后，共获得59 979个转录本（transcript），N50为3 919 bp；获得的转录本在NR、GO、KEGG、Swiss-Prot和Pfam数据库中比对和注释，共有24 487个Unigenes获注释。差异表达基因分析显示，1 112个基因在头肾中上调，包括KIT配体、早期B细胞因子1、Toll样受体1和2等免疫相关基因；2 737个基因在体肾中上调，如C-C趋化因子19和25、补体5和9、白细胞介素17受体C、RIG-I和白细胞介素27等免疫相关基因，部分抗体重链可变区编码基因和抗体轻链κ链可变区编码基因仅在体肾中表达。结果表明，黄颡鱼头肾和体肾中免疫相关基因表达具有差异性，两者造血和免疫功能具有组织特异性。研究结果有助于黄颡鱼头肾和体肾造血和免疫功能研究，可为黄颡鱼病害防治提供基础资料。 |
| 关键词: 黄颡鱼 头肾 体肾 转录组测序 |
| DOI：10.11693/hyhz20210500110 |
| 分类号:S917.4 |
| 基金项目:浙江省"水产"一流学科开放课题,11034060216号;浙江省教育厅项目,Y202044632号。 |
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| COMPARATIVE TRANSCRIPTOME ANALYSIS OF THE HEAD KIDNEY AND TRUNK KIDNEY IN ADULT YELLOW CATFISH (PELTEOBAGRUS FULVIDRACO) |
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ZHONG Ai-Hua, DAI Xiao-Xin
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College of Fishery, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| The immune system of teleost is unique because the head kidney and trunk kidney are the sites for hematopoiesis and immune response. To explore the differences of hematopoiesis and immune function between head kidney and trunk kidney, the transcriptomes of head kidney and trunk kidney of yellow catfish Pelteobagrus fulvidraco were sequenced through high-throughput sequencing technology. After trimming and filtering, clean reads were assembled into 59 979 transcripts with N50 value of 3 919 bp. A total of 24 487 Unigenes were annotated in five databases (GO, KEGG, NR, Pfam and Swissprot). Gene expression profile in the head kidney and trunk kidney were analyzed, and 3 949 differentially expressed genes (DEGs) were found. Among the DEGs, 1 112 genes were highly expressed in head kidney, including KIT ligand, Early B cell factor 1, Toll-like receptor 1, Toll-like receptor 2 etc. In total, 3 527 genes were highly expressed in trunk kidney, including C-C motif chemokine19, C-C motif chemokine 25, complement C5, C9, interleukin 17 receptor C, RIG-I, interleukin 27, etc. Some immune-related genes were expressed in trunk kidney only, such as encoded gene of immunoglobulin heavy chain and light chain. The results show that hematopoiesis and immune function between head and trunk kidney are different from each other. This study will help explore the immune defense mechanism of yellow catfish and other teleosts. |
| Key words: yellow catfish Pelteobagrus fulvidraco head kidney trunk kidney RNA sequencing |
