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| 企鹅珍珠贝矿化基因表达模式和贝壳超微结构对珍珠质层颜色影响的研究 |
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张佳谊1,2, 杨蕾1,2, 陈一1,2, 王爱民1,2, 战欣1,2
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1.南海海洋资源利用国家重点实验室 海南海口 570228;2.海南大学海洋学院 海南海口 570228
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| 摘要: |
| 为探究企鹅珍珠贝贝壳内表面珍珠质层不同区域颜色差异形成的原因，选取9个生物矿化相关基因，利用实时荧光定量PCR扩增技术对其在外套膜不同部位的表达水平进行测定，并利用扫描电子显微镜对贝壳珍珠质层不同区域的内表面及纵断面的微观结构进行观察。结果表明：有8个矿化基因在企鹅珍珠贝外套膜远端膜区和中央膜区的表达量具有显著差异（P<0.05）；贝壳珍珠质层内表面颜色不同的两个区域（生长区和中心区）表面的超微结构和纵断面文石板片厚度均存在差异。研究推测，矿化基因可能是通过在外套膜不同区域的差异表达，影响企鹅珍珠贝珍珠质的形成，进而影响珍珠质层的颜色。而文石板片厚度很可能与贝壳珍珠质层颜色无关，而是与贝壳珍珠质层分泌的早晚有关。研究为探究企鹅珍珠贝珍珠质层内表面颜色显著不同区域的形成机制提供理论基础。 |
| 关键词: 企鹅珍珠贝 珍珠质层 矿化基因 扫描电子显微镜 |
| DOI：10.11693/hyhz20210600146 |
| 分类号:S917.4 |
| 基金项目:国家自然科学基金项目，31772847号。 |
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| EFFECT OF GENE EXPRESSIONS AND SHELL ULTRASTRUCTURE ON THE COLOR OF NACREOUS LAYER IN PTERIA PENGUIN |
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ZHANG Jia-Yi1,2, YANG Lei1,2, CHEN Yi1,2, WANG Ai-Min1,2, ZHAN Xin1,2
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1.State Key Laboratory of Marine Resource Utilization in South China Sea, Haikou 570228, China;2.College of Marine Science, Hainan University, Haikou 570228, China
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| Abstract: |
| To understand the mechanism of coloration in the shell nacreous areas of Pteria penguin, real-time fluorescent quantitative PCR amplification technology was used to determine expression levels of nine biomineralization genes in different parts of the mantle, and the microstructure of shell nacreous layer with different colors were observed with the scanning electron microscope. The thickness of aragonite sheet in different shell areas was measured with the software Image J. The results reveal significant differences in the expression levels of 8 biomineralization genes among two areas of Pteria penguin mantle (P<0.05), and significantly different microstructure (P<0.05) between growth area and central area on the inner surface of nacre. The biomineralization genes may affect the structure of nacreous layer and the thickness of aragonite sheet, and the nacreous color may be impaired by the surface structure of the shell. This study provided information for understanding the mechanism of color formation of shell nacreous layer in Pteria penguin. |
| Key words: Pteria penguin nacreous layer biomineralization genes scanning electron microscope |