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引用本文:王开杰,徐永江,崔爱君,柳学周,姜燕,王滨.高体(鱼狮)(Seriola dumerili)线粒体全基因组测定及结构特征分析.海洋与湖沼,2022,53(1):120-132.
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高体(鱼狮)(Seriola dumerili)线粒体全基因组测定及结构特征分析
王开杰1,2,3, 徐永江1,2, 崔爱君1,2, 柳学周1,2, 姜燕1,2, 王滨1,2
1.中国水产科学研究院黄海水产研究所 山东青岛 266071;2.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室 山东青岛 266237;3.浙江海洋大学国家海洋设施养殖工程技术研究中心 浙江舟山 316022
摘要:
为快速有效鉴别(鱼狮)属鱼类物种、加强(鱼狮)鱼遗传多样性管理与种质资源保护,通过Illumina测序技术,获得了东海海域养殖高体(鱼狮)(Seriola dumerili)线粒体基因组全序列(16 530 bp),碱基组成为A (26.83%)、G (17.6%)、C (30.04%)和T (25.53%),A+T含量为52.36%,且非编码控制区(D-loop) A+T富含61.64%,表现明显的AT偏好性。与其他硬骨鱼一样,高体(鱼狮)线粒体基因组包含13条蛋白编码基因,22个tRNA基因,2个rRNA基因,除ND6、tRNAGlntRNAAlatRNAAsntRNACystRNATyrtRNASertRNAGlutRNAPro基因外,其余均位于H链编码;蛋白编码基因中,除COⅠ、COⅡ和ND5的起始密码子分别为ATC、ATA和ATA外,其余10个蛋白编码基因的起始密码子均为ATG,以典型的TAA和TAG为终止密码子,在ND4和Cytb中存在不完全密码子T;除tRNASer-GCT外,其余21个tRNA均为典型三叶草二级结构。比较中国和日本海域高体(鱼狮)线粒体基因组发现,CO I、CO II和ND5蛋白编码基因在起止位置、片段长度及起止密码子上存在显著差异。此外,与同属的黄条(鱼狮)(Seriola aureovittata)和五条(鱼狮)(Seriola quinqueradiata)的线粒体基因组13个蛋白编码基因进行两两对比分析,结果表明3种(鱼狮)属鱼类的蛋白编码基因的相似性在85%~100%之间。基于线粒体基因组全序列构建的系统发育树,成功将高体(鱼狮)与其他(鱼狮)属鱼类有效区分,高体(鱼狮)与长鳍(鱼狮)同属一支,亲缘关系最近;黄条(鱼狮)和五条(鱼狮)聚为一支,亲缘关系最近。
关键词:  高体(鱼狮)  线粒体基因组  序列比较分析  系统发育分析
DOI:10.11693/hyhz20210700160
分类号:S917
基金项目:国家重点研发计划项目,2019YFD0900901号,2018YFD0901204号;山东省支持青岛海洋科学与技术试点国家实验室重大科技专项,2018SDKJ0303-1号;中国水产科学研究院基本科研业务费,TD47号,2021GH05号;国家海洋水产种质资源库项目;财政部和农业农村部:国家现代农业产业技术体系资助,CARS-47号。
附件
COMPLETE SEQUENCE AND GENE ORGANIZATION OF THE MITOCHONDRIAL GENOME OF SERIOLA DUMERILI
WANG Kai-Jie1,2,3, XU Yong-Jiang1,2, CUI Ai-Jun1,2, LIU Xue-Zhou1,2, JIANG Yan1,2, WANG Bin1,2
1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;3.National Engineering Research Center For Marine Aquaculture, Zhejiang Ocean University, Zhoushan 316022, China
Abstract:
To identify the species of Seriola and strengthen the management of genetic diversity and protection of germplasm resources of the Seriola, the complete mitochondrial genome sequence of Seriola dumerili cultured in the East China Sea was successfully obtained by second-generation sequencing and bioinformatic analysis. The total length of the mitochondrial genome sequence was 16 530 bp with typical mtDNA components including 13 proteins and 22 tRNAs, 2 rRNAs and a D-Loop region, in which the A content was 26.83%, G was17.6%, C was 30.04% and T was 25.53%. Except for ND6, tRNAGln, tRNAAla, tRNAAsn, tRNACys, tRNATyr, tRNASer, tRNAGlu, and tRNAPro, all the other genes were encoded on the H-strand. The A+T content of the complete mitochondrial genome sequence and the protein coding gene were 52.36% and 51.72%, respectively. Among the 22 tRNA genes, except for tRNAser-GCT, the other 21 tRNA genes contained typical secondary structure of clover. The results show that the similarity of 13 protein coding genes in the whole mitochondrial genome of the three species was over 85% to 100%. The genes encoding CO I, CO II, and ND5 showed difference at the start point, length, and starting codon between Chinese and Japanese S. dumerili. Furthermore, the phylogenetic tree was constructed with the complete mitochondrial genome sequences of four species from Seriola genus. The phylogenetic tree showed that S. aureovittata and S. quinqueradiata were clustered in one branch, and the phylogenetic tree of S. dumerili and S. rivoliana belonged to the same proximal branch. The phylogenetic relationship between S. aureovittata and S. quinqueradiata, and between S. dumerili and S. rivoliana are the closest.
Key words:  Seriola dumerili  mitochondrial genome  sequence analysis  phylogenetic tree
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