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引用本文:史恬恬,谢超,张家玮,李远慧,朱亚猛,吴玉婷,周卓颖,虞舟.基于低压变频电场技术对带鱼(Trichiurus lepturus)保鲜过程中微生物群落影响分析.海洋与湖沼,2022,53(1):133-140.
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基于低压变频电场技术对带鱼(Trichiurus lepturus)保鲜过程中微生物群落影响分析
史恬恬1, 谢超1, 张家玮1, 李远慧1, 朱亚猛1, 吴玉婷1, 周卓颖1, 虞舟2
1.浙江海洋大学食品与药学学院 浙江舟山 316022;2.舟山汇丰冷藏物流发展有限公司 浙江舟山 316002
摘要:
带鱼(Trichiurus lepturus)在低温条件运输和贮藏过程中,易受微生物和内源性蛋白酶的影响而产生胺类和醛类物质导致腐败。为了研究低压变频电场对微冻带鱼在贮藏过程中的微生物群落特征,采用Miseq基因组测序技术,对贮藏至40 d的舟山带鱼的微生物群落进行碱基信息测序。将产生的碱基序列通过OTU聚类、多样性分析、相关性分析等手段分析了影响带鱼保鲜效果的微生物群落组成和发育信息。在此基础上,研究了不同强度电场(0,2,2.5,3 kV/m)处理对带鱼微生物群落组成的影响。根据菌属相对丰度结果,推测希瓦氏菌属(Shewanella)、假单胞菌属(Pseudomonas)、紫色杆菌属(Janthinobacterium)是带鱼微生物腐败主要致腐菌。结果表明,40 d后,经3 kV/m处理的样品其OTU6嗜冷菌属和OTU13假单胞菌属c的相对丰度均小于1%,而且OTU7、OTU14、OTU8、OTU9等菌属未被OTU聚类。结果表明,经3 kV/m电场强度的低压静电场处理的抑菌保鲜效果最好,表现为在贮藏过程中杂菌种类最少,样本微生物群落物种丰度和多样性均最低,腐败程度最低。
关键词:  带鱼(Trichiurus lepturus)  低压变频电场  微生物  高通量测序
DOI:10.11693/hyhz20210800189
分类号:TS254
基金项目:“十三五”国家重点研发计划重点专项,2019YFD0901604号;舟山市科技计划项目,2020C12016号;舟山市普陀区科技计划项目,2020JH102号。
附件
EFFECT OF LOW-VOLTAGE ALTERNATING FREQUENCY ELECTRIC FIELD ON MICROBIAL COMMUNITY OF HAIRTAIL TRICHIURUS LEPTURUS DURING PRESERVATION
SHI Tian-Tian1, XIE Chao1, ZHANG Jia-Wei1, LI Yuan-Hui1, ZHU Ya-Meng1, WU Yu-Ting1, ZHOU Zhuo-Ying1, YU Zhou2
1.Zhejiang Ocean University College of Food and Medicine, Zhoushan 316022, China;2.Zhoushan HSBC Cold Storage Logistics Development, Co. Ltd., Zhoushan 316002, China
Abstract:
To study the effect of low-voltage variable-frequency electric field on the microbial community characteristics of microfrozen hairtail (Trichiurus lepturus) during storage, Miseq genome sequencing technology was used to sequence the base information of the microbial community of Zhoushan hairtail stored for 40 days. The microbial community composition and development information affecting the preservation effect of hairtail were analyzed by OTU clustering, diversity analysis, and correlation analysis. On this basis, the effects of electric field in different intensities (0, 2, 2.5, 3 kV/m) on the microbial community composition of hairtail were studied. According to the results of relative abundance of bacteria, we speculated that Shewanella, Pseudomonas, and Janthinobacterium were the main spoilage bacteria of hairtail microbial spoilage. The results show that after 40-day storage, the relative abundances of OTU6 psychrophilic bacteria and OTU13 Pseudomonas C were less than 1%, and OTU7, OTU14, OTU8, OTU9 and other bacterial genera were not clustered by OTU. Specifically, the antibacterial and fresh-keeping effect of low-voltage electrostatic field in 3 kV/m intensity was the best, under which the species of miscellaneous bacteria were the least, the species abundance and diversity of microbial community were the lowest, and the degree of corruption was the lowest.
Key words:  hairtail Trichiurus lepturus  low-voltage electrostatic field  microorganism  high-throughput sequencing
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