| 引用本文: | 栗洋洋,贾梦雪,王锦乙,吴紫琪,羊妍珂,严小军,廖智,何建瑜.厚壳贻贝(Mytilus coruscus)中抗生素耐受细菌多样性研究.海洋与湖沼,2022,53(2):405-413. |
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| 摘要: |
| 抗生素的滥用是当前水产养殖业发展及食品安全面临的重大威胁。贻贝体内含有丰富的附生菌群,但目前尚不清楚贻贝附生菌群在抗生素残留背景下的动态变化和耐受性特征。为此进行了厚壳贻贝中抗生素耐受细菌多样性研究。将厚壳贻贝暴露于含有青霉素、链霉素和卡那霉素的养殖水体中,之后采用超声法获取含贻贝软体部组织表面附着菌群的超声液,经Zobell 2216E液体培养基培养后,将组织超声液和培养液一并经16S rDNA高通量测序来分析厚壳贻贝微生物的群落组成。结果表明,超声处理后获得的微生物主要为拟杆菌门(Bacteroidetes)、变形菌门(Proteobacteria)和疣微菌门(Verrucomicrobia)。多重抗生素的处理可以显著下降贻贝体内微生物的OTU数,对微生物群落结构有明显影响。经264h恢复养殖后,在门水平上观察到贻贝体内微生物群落可以恢复重建,表明其具有一定的韧性。研究结果将为今后进一步探索宿主相关微生物群落构建机制及特殊抗性微生物奠定基础。 |
| 关键词: 厚壳贻贝 抗生素耐受性 细菌 16S rDNA |
| DOI：10.11693/hyhz20210900199 |
| 分类号:S917.1 |
| 基金项目:国家自然科学基金项目,42020104009号;浙江省“省属高校基本科研业务费”项目,2021J001号;国家级大学生创新创业训练计划,202110340029号;浙江省大学生科技创新活动计划暨新苗人才计划,2021R411005号。 |
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| DIVERSITY OF ANTIBIOTIC RESISTANT BACTERIA IN MYTILUS CORUSCUS |
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LI Yang-Yang, JIA Meng-Xue, WANG Jin-Yi, WU Zi-Qi, YANG Yan-Ke, YAN Xiao-Jun, LIAO Zhi, HE Jian-Yu
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Laboratory of Marine Biology Protein Engineering, College of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| The abuse of antibiotics is a major threat to the aquaculture industry and food safety. Mussels are rich in symbiotic bacteria. However, the dynamic variation and tolerance characteristics of the microbiota exposed to residual antibiotics remain poorly understood. To fill the knowledge gap, the diversity of antibiotic resistant bacteria in Mytilus coruscus was carried out. M. coruscus were exposed to seawater containing penicillin, streptomycin, and kanamycin. The ultrasonic solution containing symbiotic bacteria colonized on the surface of M. coruscus soft part was obtained ultrasonically. The ultrasonic solution was then cultured in Zobell 2216E liquid medium and the culture solution was subjected to 16S rDNA high-throughput sequencing. Results show that the microorganisms were mainly Bacteroidetes, Proteobacteria, and Verrucomicrobia. The joint effect of multiple antibiotics could significantly reduce the OTUs of microbiome in M. coruscus with an obvious impact on the microbial community structure. After recovering for 264 h, the microbial community in M. coruscus was restored and reconstructed at phylum level, reflecting its resilience to a certain extent. This study lays foundation for further exploration of the assembly principles of host-associated microbiota and special resistant microbes in the future. |
| Key words: Mytilus coruscus antibiotic resistance bacteria 16S rDNA |
