| 引用本文: | 李忠琴,邹文政,张光明,杨求华,庄若飞,乔欣君,江兴龙.植物乳杆菌(Lactobacillus plantarum)对云龙石斑鱼生长、抗氧化性能及血清生化水平的影响.海洋与湖沼,2022,53(2):457-465. |
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| 植物乳杆菌(Lactobacillus plantarum)对云龙石斑鱼生长、抗氧化性能及血清生化水平的影响 |
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李忠琴1, 邹文政1, 张光明1, 杨求华2, 庄若飞3, 乔欣君3, 江兴龙1
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1.集美大学水产学院 鳗鲡现代产业技术教育部工程研究中心 福建厦门 361021;2.福建省水产研究所 福建厦门 361012;3.厦门惠盈动物科技有限公司 福建厦门 361021
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| 摘要: |
| 植物乳杆菌(Lactobacillus plantarum)常作为饲料添加剂,可抑制鱼类病原菌生长,增加机体对饲料的消化和吸收。探讨了饲喂不同添加量的植物乳杆菌对云龙石斑鱼(Epinephelus moara♀×E.lanceolatus♂)生长性能、肠道消化酶活性、肠道和肝脏抗氧化能力、血清生化水平的影响,以期为植物乳杆菌在其健康养殖中的应用提供依据。结果显示,随植物乳杆菌的饲喂量增加,云龙石斑鱼的终末均重(FAW)、增重率(WGR)、特定生长率(SGR)和肠道中淀粉酶(AMS)、脂肪酶(LPS)和糜蛋白酶(CHY)、总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、还原型谷胱甘肽(GSH)、超氧化物歧化酶(SOD)的变化均呈现先升高后降低的趋势。而随着植物乳杆菌的饲喂量增加,云龙石斑鱼的血清中总蛋白(TP)、白蛋白(Alb)、胆固醇(TC)、碱性磷酸酶(AKP)、酸性磷酸酶(ACP)、超氧化物歧化酶(SOD)、丙二醛(MDA)均略降低,甘油三酯(TG)略升高,但与对照组差异不明显(P>0.05)。当添加量为1×105CFU/g时,云龙石斑鱼的各生长指标和抗氧化性能均优于对照组,其中增重率显著提高了19.8%(P<0.05),肠道T-AOC、SOD分别显著升高了93.2%、2.9%(P<0.05),肝脏MDA显著降低了32.7%(P<0.05)。当添加量为1×107CFU/g时,云龙石斑鱼的终末均重、增重率、特定生长率均显著高于对照组和其余添加组(P<0.05);饲料系数显著低于对照组(P<0.05);肠道的AMS、LPS、CHY活性分别显著提高了13.0%、166.9%、61.1%(P<0.05);但肠道和肝脏的抗氧化性能变化不明显,仅肝脏MDA显著降低了23.9%(P<0.05)。由此表明,饲料中添加一定量的植物乳杆菌可以提高肠道消化能力和抗氧化能力,促进云龙石斑鱼的生长,但对其血清生化水平无显著性影响,不会对鱼体代谢产生不良作用。 |
| 关键词: 植物乳杆菌 云龙石斑鱼 抗氧化性能 血清生化水平 |
| DOI：10.11693/hyhz20210900215 |
| 分类号:Q956; S965 |
| 基金项目:福建省科技计划项目,2021N0014号;国家重点研发计划,2020YFD0900102号;财政部和农业农村部:国家现代农业产业技术体系资助,CARS-46号;福建省海洋经济发展补助资金项目,FJHJF-L-2020-4号;厦门南方海洋研究中心项目,19CZP003HJ07号;集美大学培育基金,ZP2021003号。 |
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| EFFECTS OF LACTOBACILLUS PLANTARUM-ENRICHED DIET ON GROWTH PERFORMANCE, ANTIOXIDANT ABILITY, AND SERUM BIOCHEMICAL LEVEL OF EPINEPHELUS YUNLONG GROUPER |
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LI Zhong-Qin1, ZOU Wen-Zheng1, ZHANG Guang-Ming1, YANG Qiu-Hua2, ZHUANG Ruo-Fei3, QIAO Xin-Jun3, JIANG Xing-Long1
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1.Engineering Research Center of the Modern Technology for Eel Industry of Ministry of Education, Fisheries College, Jimei University, Xiamen 361021, China;2.Fujian Fisheries Research Institute, Xiamen 361012, China;3.Xiamen Huiying Animal Technology Co. Ltd., Xiamen 361021, China
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| Abstract: |
| The effects of Lactobacillus plantarum supplementation on the growth, intestinal digestive enzyme activity, intestinal and liver antioxidant capacity, and serum biochemical level of grouper (Epinephelus moara♀×Epinephelus lanceolatus♂) were studied. A basal diet was enriched by adding L. plantarum in doses of 0 (the control), 1×105, 1×107, and 1×109 CFU/g representing different groups, respectively. Results show that the final average weight (FAW), weight gain rate (WGR), specific growth rate (SGR), amylase (AMS), lipase (LPS), chymotrypsin (CHY), total antioxidant capacity (T-AOC) and catalase (CAT), reduced glutathione (GSH), and superoxide dismutase (SOD) in the intestine of grouper increased first and then decreased with the increase in dose of L. plantarum addition. The total protein in serum (TP), albumin (ALB), cholesterol (TC), alkaline phosphatase (AKP), acid phosphatase (ACP), superoxide dismutase (SOD) and malondialdehyde (MDA) decreased slightly, and triglyceride (TG) increased slightly, but no significant difference in all the indices compared to those of the control group (P>0.05). In 1×105 CFU/g dose group, the growth performance and antioxidant ability were better than those of control group, and WGR was significantly increased by 19.8% (P<0.05). T-AOC and SOD of intestine increased significantly by 93.2%, and 2.9% (P<0.05) respectively, and MDA of liver decreased significantly by 32.7% (P<0.05). In dose group of 1×105 CFU/g, the FAW, WGR, and SGR indicative of growth performance of grouper were significantly higher than those in the control group and other dose groups (P<0.05). The feed coefficient (FCR) was significantly lower than that of the control group (P<0.05). The activities of AMS, LPS, and CHY in intestine were significantly increased respectively by 13.0% (P<0.05), 166.9% (P<0.05), and 61.1% (P<0.05), but the antioxidant performance of grouper intestine and liver had no significant change, only MDA of liver decreased significantly by 23.9% (P<0.05). Therefore, a certain dose of L. plantarum supplementation on diet could improve the intestinal digestion and antioxidant ability and promote the growth of grouper. However, it has no significant effect on the serum biochemical level of grouper and nor any adverse effects on the metabolism of grouper. This study provides basic data for the development and application of probiotics in the culture of grouper in a green and healthy way. |
| Key words: Lactobacillus plantarum Yunlong grouper(Epinephelus moara♀×Epinephelus lanceolatus)♂ antioxidant ability serum biochemical level |
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