引用本文:	唐亚鹏,董世瑞,田吉腾,周茜,王印庚,张天时.凡纳滨对虾(Litopenaeus vannamei)循环水养殖系统中养殖效果及菌群结构和功能研究.海洋与湖沼,2022,53(2):466-474.

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*凡纳滨对虾(Litopenaeus vannamei)循环水养殖系统中养殖效果及菌群结构和功能研究*
唐亚鹏^1,2, 董世瑞³, 田吉腾¹, 周茜^1,2, 王印庚^1,2, 张天时^1,2
1.中国水产科学研究院黄海水产研究所山东青岛 266071;2.青岛海洋科学与技术试点国家实验室海洋渔业科学与食物产出过程功能实验室山东青岛 266237;3.天津市食品生物技术重点实验室天津商业大学生物技术与食品科学学院天津 300134

摘要:

循环水养殖可有效减少病原体的入侵,是我国水产养殖业发展的重要方向。为完善凡纳滨对虾(Litopenaeus vannamei)循环水养殖系统,采用生态调控、16S rRNA高通量测序、宏基因组学分析与数理统计等方法,系统分析了对虾循环水养殖系统中水质指标变化与菌群结构和基因功能的关系。结果显示,养殖池水质指标包括温度、溶解氧、盐度、pH以及氨氮0.11~1.16mg/L,亚硝酸氮0.10~0.66mg/L,硝酸氮0.84~35.40mg/L均在安全范围内;经过63d养殖,凡纳滨对虾平均体重达到11.78g左右,产量为3.28kg/m³左右,存活率为69.59%左右。菌群结构与功能分析结果显示:在开始运行期与水质变化平稳期生物滤池中菌群结构差异较大。在开始运行期海杆菌属(31.37%)占绝对优势,而在平稳期则以分枝杆菌属(6.65%)、分枝菌酸杆菌属(6.39%)、食烷菌属(5.21%)、海杆菌属(3.36%)、中慢生根瘤菌属(2.30%)、红杆菌属(1.34%)、副球菌属(1.29%)等反硝化细菌和硝化杆菌属(1.17%)占据优势。通过比对KEGG数据库发现涉及碳水化合物代谢和氨基酸代谢的蛋白数目最多,说明异养反硝化菌需要利用多种碳源来执行反硝化功能。

关键词: 循环水养殖凡纳滨对虾高通量测序宏基因组学菌群结构

DOI：10.11693/hyhz20210800175

分类号:

基金项目:国家重点研发计划重点专项,2019YFD0900403号;国家重点研发计划重点专项,2019YFD0900104。

附件

STUDY ON STRUCTURE AND FUNCTION OF BACTERIA COMMUNITY AND THE COUTURE EFFECT OF RECIRCULATING AQUACULTURE SYSTEM FOR LITOPENAEUS VANNAMEI

TANG Ya-Peng^1,2, DONG Shi-Rui³, TIAN Ji-Teng¹, ZHOU Qian^1,2, WANG Yin-Geng^1,2, ZHANG Tian-Shi^1,2

1.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology (Qingdao), Qingdao 266237, China;3.Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China

Abstract:

To improve the performance of recirculating aquaculture system (RAS) of Litopenaeus vannamei, relationships between the changes of water quality indexes and the structure of bacterial community and gene function in the RAS were analyzed by ecological regulation, 16S rRNA high-throughput sequencing, metagenomic analysis, and mathematical statistics. Results show that all the water quality indexes, including temperature, dissolved oxygen, salinity, pH, ammonia nitrogen, nitrite nitrogen, and nitrate nitrogen could meet the safety requirements. After 63 days of culture, the average weight of L. vannamei reached 11.78 g, the yield was 3.28 kg/m³, and the survival rate was 69.59%. The results of microbial community structure and function analysis show that there were significant differences in microbial community structure between the start-up period and the stabilized period of water quality. In the start-up period, Marinobacter (31.37%) was dominant, while in the stabilized period, the denitrifying bacteria including Mycobacterium (6.65%), Mycobacterium (6.39%), Alcanivorax (5.21%), Marinobacter (3.36%), Mesorhizobium (2.30%), Rhodobacter (1.34%), Rhodobacter (1.42%), Paracoccus (1.29%), and Nitrobacter (1.17%) took over. By comparing against the KEGG database, we found that carbohydrate metabolism and amino acid metabolism occupied the largest number of proteins in the metabolic pathway, indicating that heterotrophic denitrifiers require multiple carbon sources to perform denitrification function.

Key words: recirculating aquaculture system Litopenaeus vannamei high throughput sequencing metagenomics microbial community structure