| 摘要: |
| 虎龙杂交斑(Epinephelus lanceolatus♂×E.fuscoguttatus♀)是我国重要的石斑鱼养殖品种,为研究其对20种常见氨基酸的不同嗅觉反应,筛选出对虎龙杂交斑嗅觉具有高效刺激性的单体氨基酸,通过嗅上皮水下嗅电位法(electro-olfactogram,EOG)得出了虎龙杂交斑对20种常见氨基酸的嗅觉反应差异和对5种氨基酸的嗅觉反应阈值,并讨论了氨基酸分子结构与嗅觉刺激效果之间的关系。结果表明:氨基酸的种类和浓度均对虎龙杂交斑嗅觉刺激反应有影响,虎龙杂交斑对含5~6个碳原子、无支链的中性脂肪族含硫氨基酸反应强烈,一个例外是对含3个碳原子的半胱氨酸有较强反应。10–2 mol/L的蛋氨酸刺激液对虎龙杂交斑嗅觉的刺激性最强,10–6 mol/L脯氨酸刺激液对虎龙杂交斑嗅觉的刺激性最弱。虎龙杂交斑嗅觉反应幅值(y)随5种氨基酸刺激液浓度对数值(x)的增大而呈指数升高趋势,在试验中虎龙杂交斑嗅觉反应幅值没有随5种氨基酸刺激液浓度对数值的增大而达到平台期。从反应阈值中反应幅值与氨基酸刺激液浓度的关系方程式可得出反应阈值浓度为10–10~10–6 mol/L。研究结果有助于为虎龙杂交斑诱食剂和饲料配制的研究提供依据。 |
| 关键词: 虎龙杂交斑(Epinephelus lanceolatus♂×E. fuscoguttatus♀) 诱食剂 氨基酸 电生理学 嗅觉 嗅上皮水下嗅电位法 |
| DOI：10.11693/hyhz20211100297 |
| 分类号: |
| 基金项目:浙江省重点研发计划项目,2021C04016号;舟山市科技计划项目,2015C41003号。 |
附件 |
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| OLFACTORY ELECTROPHYSIOLOGICAL RESPONSES OF HYBRID GROUPER (EPINEPHELUS LANCEOLATUS♂×E. FUSCOGUTTATUS♀) TO 20 GENERAL AMINO ACIDS |
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XU Wen-Dan1, WANG Ji-Teng2, HAN Tao2, YAN Xiao-Jun1
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1.Marine Science and Technology College, Zhejiang Ocean University, Zhoushan 316022, China;2.College of Fisheries, Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| To explore the different olfactory responses of hybrid grouper (Epinephelus lanceolatus♂×E. fuscoguttatus♀) to 20 general amino acids, and to screen the single amino acids that are highly stimulating to hybrid grouper, the differences of olfactory responses to 20 general amino acids and the olfactory response thresholds to five amino acids were determined in the electro-olfactogram (EOG). Relationship between the molecular structure of amino acids and the effect of olfactory stimulation was clarified. Results show that the type and concentration of amino acids had an effect on hybrid grouper olfactory stimulation. Hybrid grouper had a strong reaction to neutral aliphatic sulfur-containing amino acids with 5~6 carbon atoms and no branched chain, with an exception of strong reaction to cysteine containing 3 carbon atoms. In an experiment of this study, 10–2 mol/L methionine stimulation showed the strongest stimulation effect to the hybrid grouper’s olfactory, while 10–6 mol/L proline stimulation displayed the weakest stimulation effect. The amplitude of hybrid grouper olfactory response increased exponentially with the increase of the logarithmic concentration of the 5 amino acids stimulation. In the experiments of this study, the amplitude of olfactory response of hybrid grouper did not reach the plateau with the increase of logarithmic concentration of 5 amino acids. Relationship between the reaction amplitude and the concentration of amino acids stimulation in the reaction threshold satisfies a correlation equation, and the threshold concentration was 10–10~10–6 mol/L. This study provide a basis for the formulations of food attractant and feed for hybrid grouper. |
| Key words: hybrid grouper (Epinephelus lanceolatus♂×E. fuscoguttatus♀) feeding stimulant amino acids olfactory electrophysiology electro-olfactogram |
