| 引用本文: | 赵楠楠,崔彦婷,王忠凯,王聪,张志豪,邓之通,赵瑞阳,孙金凤,王仁杰,李玉全.投喂频率对凡纳滨对虾(Litopenaeus vannamei) PI3K信号通路及糖代谢相关酶基因表达的影响.海洋与湖沼,2022,53(5):1189-1196. |
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| 投喂频率对凡纳滨对虾(Litopenaeus vannamei) PI3K信号通路及糖代谢相关酶基因表达的影响 |
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赵楠楠, 崔彦婷, 王忠凯, 王聪, 张志豪, 邓之通, 赵瑞阳, 孙金凤, 王仁杰, 李玉全
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青岛农业大学海洋科学与工程学院 山东青岛 266109
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| 摘要: |
| 适宜的投喂频率能提高对虾的代谢和免疫能力,加快生长,实现提质增效的目标。为探讨不同投喂频率对凡纳滨对虾(Litopenaeus vannamei)糖代谢及相关信号通路的影响,以(7.6±1.0) g的凡纳滨对虾为实验材料,设置2、3、4、6次/d,共4个投喂频率处理组,每组设3个平行,实验持续14 d,实验结束时取样,测定PI3K信号通路相关因子PI3K、Akt、HIF-1α、mTOR,代谢酶基因中HK、PFK、GAPDH、PK、FBP、PEPCK,葡萄糖转运蛋白GLUT1、GLUT2 mRNA表达水平的变化。结果发现:随着投喂频率的增加, PI3K信号通路关键基因表达的水平显著上升(P<0.05),其中6次/d投喂组PI3K、Akt、HIF-1α和mTOR表达水平显著高于2次/d投喂组(P<0.05), 4次/d投喂组mTOR表达水平显著高于2次/d投喂组(P<0.05);代谢关键酶基因HK、PFK、GAPDH、PK、FBP、PEPCK的表达水平随投喂频率的增加而增加,其中6次/d投喂组糖酵解HK、PFK、GAPDH、PK酶基因表达水平显著高于2次/d投喂组(P<0.05), 3、4和6次/d投喂组糖异生酶FBP表达水平显著高于2次/d投喂组(P<0.05),葡萄糖转运蛋白GLUT2在2、3、6次/d投喂频率处理组显著高于4次/d频率投喂组(P<0.05)。得出结论认为较高的投喂频率可以通过激活凡纳滨对虾糖代谢酶基因、糖转运蛋白及PI3K信号通路的表达来提高糖代谢水平。 |
| 关键词: 凡纳滨对虾 投喂频率 糖代谢酶 糖转运蛋白 PI3K信号通路 |
| DOI：10.11693/hyhz20211200336 |
| 分类号:Q789;S968 |
| 基金项目:山东省虾蟹产业技术体系,SDAIT-15-011号;山东省农业科技资金(园区产业提升工程)项目,2019YQ003号;国家自然科学基金项目,31802269号;山东省自然科学基金项目,ZR2019BC013号。 |
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| EFFECTS OF FEEDING FREQUENCY ON EXPRESSION OF GENES RELATED TO METABOLISM AND PI3K SIGNALING PATHWAY IN LITOPENAEUS VANNAMEI |
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ZHAO Nan-Nan, CUI Yan-Ting, WANG Zhong-Kai, WANG Cong, ZHANG Zhi-Hao, DENG Zhi-Tong, ZHAO Rui-Yang, SUN Jin-Feng, WANG Ren-Jie, LI Yu-Quan
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School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
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| Abstract: |
| To investigate the effects of different feeding frequencies on glucose metabolism and related signaling pathways in Litopenaeus vannamei,the PI3K signaling pathways (PI3K, Akt, HIF-1α,and mTOR),metabolic enzyme (HK,PFK, GAPDH,PK, FBP,and PEPCK),and glucose transporters(GLUT1 and GLUT2) mRNA expression in the hepatopancreas were determined.Shrimps[(7.6±1.0) g]were randomly distributed in four-frequency feeding treatments (2×/d,3×/d,4×/d,and 6×/d) in triplicate.The experiment lasted for 14 days.Results show that the expression levels of key genes in PI3K signaling pathway increased significantly at higher frequencies (P<0.05).The expression levels of PI3K, Akt, HIF-1α,and mTOR in 6×/d were significantly higher than those of 2×/d (P<0.05),and the expression level of mTOR in 4×/d was significantly higher than those of 2×/d (P<0.05).The expression levels of HK, PFK, GAPDH, PK,FBP,and PEPCK increased with the increase in feeding frequency.The expression levels of glycolytic genes HK, PFK, GAPDH,and PK in 6×/d group were significantly higher than those of 2×/d (P<0.05).The expression levels of gluconeogenic enzyme FBP in 3×/d,4×/d,and 6×/d groups were significantly higher than those of 2×/d (P<0.05).Glucose transporter GLUT2in 2×/d,3×/d,and 6×/d was significantly higher than those of 4×/d (P<0.05).Therefore,higher feeding frequency could improve glucose metabolism by activating glucose metabolism enzyme,glucose transporter and PI3K signaling pathway in L.vannamei. |
| Key words: Litopenaeus vannamei feeding frequency glucose metabolism enzyme glucose transporter PI3K signaling pathway |
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