| 摘要: |
| 为延长三疣梭子蟹(Portunus trituberculatus)在捕捞后的存活时间,在优化保活温度和充氧量的基础上,再采用基于核磁共振(NMR)的代谢组学技术,比较分析了三疣梭子蟹前鳃代谢谱在充氧和不充氧保活过程中的变化。结果发现, 12 ℃下的三疣梭子蟹存活率最高, 80%的三疣梭子蟹能存活3.5 d。加大充氧量能提高三疣梭子蟹的存活率, 3.6 L氧气可满足一只三疣梭子蟹成蟹存活到3 d以上。因此,保活三疣梭子蟹的适宜温度和充氧量分别是12 ℃和3.6 L。不充氧保活会导致三疣梭子蟹前鳃中乳酸、丙氨酸和葡萄糖等代谢物的快速积累,但充氧保活可抑制这些代谢物的变化,表明氧气通过缓解三疣梭子蟹前鳃的缺氧和渗透压失衡,从而有效延长三疣梭子蟹的存活时间。研究结果为三疣梭子蟹的保活运输提供可借鉴的方法和理论参考。 |
| 关键词: 三疣梭子蟹 保活 氧气 核磁共振 代谢组学 |
| DOI：10.11693/hyhz20220100025 |
| 分类号:Q785;S942.5 |
| 基金项目:宁波市科技创新2025重大专项,2019B10010号;财政部和农业农村部:国家现代农业产业技术体系资助。 |
附件 |
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| THE OPTIMAL TEMPERATURE AND SUPPLEMENTED OXYGEN AMOUNT FOR LIVE PORTUNUS TRITUBERCULATUS AND THE METABOLIC RESPONSE OF ITS ANTERIOR GILL |
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ZENG Ting-Lan1, CHEN Yan1, MU Chang-Kao1,2, WANG Chun-Lin1,2, SHI Ce1,2, YE Yang-Fang1
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1.School of Marine Sciences, Ningbo University, Ningbo 315832, China;2.Key Laboratory of Applied Marine Biotechnology, Ningbo University, Chinese Ministry of Education, Ningbo 315832, China
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| Abstract: |
| To extend the post-capture survival time of swimming crab Portunus trituberculatus,the temperature and oxygen amount to keep swimming crab alive in a transport bag were optimized.The metabolic changes of anterior gills of the crabs kept in transport bag with or without oxygen supply were investigated using a nuclear magnetic resonance (NMR)-based metabolomics technique.Results showed that the highest survival rate of the crab occurred in 12℃,under which 80% of them could survive up to 3.5 days.The survival rate of swimming crab increased with the supplemented oxygen amount;3.6 L oxygen was enough to keep an adult swimming crab alive for more than three days.Therefore,the optimal conditions to keep a swimming crab alive are 12℃ and 3.6 L oxygen supply,respectively.Moreover,metabolites such as lactate,alanine,and glucose were rapidly accumulated in the anterior gill of the crab exposed to air.However,oxygen could inhibit the changes of these metabolites,indicating that oxygen can extend the survival time of swimming crab by mitigating the oxygen deficit and osmotic imbalance in the anterior gill.This study provides a potential method and theoretical reference for keeping swimming crab alive during shipping. |
| Key words: Portunus trituberculatus keeping alive oxygen nuclear magnetic resonance metabolomics |
