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引用本文:张玉敏,王玉萌,宫相忠.应用响应曲面法优化萱藻丝状体单室孢子囊发育条件.海洋与湖沼,2023,54(2):483-492.
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应用响应曲面法优化萱藻丝状体单室孢子囊发育条件
张玉敏, 王玉萌, 宫相忠
中国海洋大学海洋生命学院 山东青岛 266003
摘要:
为优化萱藻丝状体单室孢子囊发育条件,提高孢子囊比例,研究以萱藻丝状体为材料,在单因素试验的基础上,应用响应曲面法对丝状体单室孢子囊的发育条件进行优化。结果显示,诱导萱藻丝状体单室孢子囊发育的最佳条件为:温度17.67℃,光照强度30.32 μmol/(m2·s),外加氮浓度37.37 mg/L,外加磷浓度10.63 mg/L。在该条件下诱导培养20 d,丝状体孢子囊比例为48.92%±5.58%,约为对照组的1.41倍。试验结果与理论预测值的相对误差小于5%,模型可靠。为评价优化培养后萱藻丝状体孢子囊的发育情况,对丝状体进行了阴干刺激(17℃,2 h),结果显示,优化培养后的丝状体游孢子放散量(3.33×106ind./g FW)约为对照组(1.67×106ind./g FW)的1.99倍。研究表明,在萱藻丝状体单室孢子囊诱导过程中,对诱导环境条件进行有目的性的调控,不仅可以提高孢子囊比例,还可以促进孢子囊发育和游孢子放散的同步性。
关键词:  萱藻  丝状体  响应曲面法  孢子囊  发育
DOI:10.11693/hyhz20220600167
分类号:S968.4
基金项目:山东省重点研发计划,2016GSF115042号。
附件
OPTIMIZATION OF DEVELOPMENTAL CONDITIONS OF UNICELLULAR SPORANGIA OF FILAMENTS OF SCYTOSIPHON LOMENTARIA BY RESPONSE SURFACE METHODOLOGY (RSM)
ZHANG Yu-Min, WANG Yu-Meng, GONG Xiang-Zhong
College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Abstract:
To optimize the conditions of the development of unicellular sporangia of Scytosiphon lomentaria and increase the proportion of sporangia, based on single-factor experiment, the response surface methodology was used to optimize the cultural conditions of unilocular sporangia of filamentous. Temperature, light intensity, added nitrogen concentration, and added phosphorus concentration were tested and the optimal conditions were determined as 17.67℃, 30.32 μmol/(m2·s), 37.37 mg/L, and 10.63 mg/L, respectively. Under the optimal condition of induction culture for 20 days, the proportion of sporangia was 48.92%±5.58%, higher than that of the control group (34.80%±4.29%) (P<0.05). In addition, the relative error between the experimental results and the theoretical model prediction value is less than 5%, and thus the model was proven reliable. To evaluate the development of unicellular sporangia of filaments in the optimized culture, the filaments were stimulated in shade (17℃, 2 h), after which the number of zoospores released in the experimental group was 3.33×106 ind./g FW, higher than that of the control group (1.67×106 ind./g FW) (P<0.05). During the induction of unicellular sporangia of S. lomentaria, the induction environmental conditions could be controlled purposely, which could not only increase the proportion of sporangia, but also promote the synchronization of the development of unicellular sporangia and the release of zoospores.
Key words:  Scytosiphon lomentaria  filaments  response surface methodology  sporangia  development
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