| 摘要: |
| 氨基脲(semicarbazide, SEM)作为新型环境污染物已在海洋、食品和人体尿液中检出, 其对环境和生物体可能带来的影响越来越受到人们的关注。研究采用1、10、100、1 000 µg/L的SEM急性暴露96 h的成年雄性斑马鱼, 对性腺指数、17β-雌二醇(E2)和睾酮(T)的含量、性激素合成酶以及HPG轴相关的基因相对表达量进行测定, 探究SEM急性暴露对雄性斑马鱼的内分泌干扰效应。结果表明, 低剂量1 µg/L SEM急性暴露通过抑制下丘脑中HPG轴起始因子s型促性腺激素释放激素(sGnRH) sGnRHmRNA相对表达量,进一步抑制促卵泡激素(FSH) FSHβ、促黄体激素(LH)LHβ、细胞色素P450 19B (CYP19B) CYP19B基因相对表达量, 中高剂量10、100、1 000 µg/L的SEM急性暴露下调下丘脑sGnRHmRNA的表达却上调c型促性腺激素释放激素(cGnRH) cGnRH、CYP19B的表达; 而不同浓度的SEM暴露均会下调精巢内3β-羟类固醇脱氢酶(3β-HSD) 3β-HSD、细胞色素P450 17 (CYP17) CYP17、细胞色素P450 11A (CYP11A) CYP11AmRNA的表达水平, 上调细胞色素P450 19A (CYP19A) CYP19A基因的相对表达量, 进而抑制T的合成, 降低E2的水平。综上, 我们认为SEM通过干扰HPG轴抑制类固醇合成酶的表达, 进而降低雄性斑马鱼的性激素水平, 从而发挥潜在的内分泌干扰效应。 |
| 关键词: 氨基脲 性激素 HPG轴 内分泌干扰效应 斑马鱼 |
| DOI：10.11693/hyhz20220900234 |
| 分类号:Q142.8 |
| 基金项目:山东省自然科学基金重点项目,ZR2014DZ001号；沧州市科技支撑计划项目,183304001号 |
附件 |
|
| ENDOCRINE DISRUPTING EFFECTS OF ACUTE EXPOSURE TO SEMICARBAZIDE ON MALE ZEBRAFISH (DANIO RERIO |
|
GUO Lin-Lin1, LIANG Meng-Meng1,2
|
|
1.Department of Life Science, Cangzhou Normal University, Cangzhou 061001, China;2.Hebei Aquaculture Microbial Agent Innovation Technology Center, Cangzhou 061001, China
|
| Abstract: |
| Semicarbazide (SEM), a new environmental pollutant, has been detected in the marine, food, and human urine. Its effects on the environment and living organisms have attracted more and more attention. In this study, adult male zebrafish were exposed to 1, 10, 100, 1 000 µg/L SEM for 96 h. The gonadosomatic index, 17 β-estradiol (E2) and testosterone (T) concentrations, steroidogenic protein, and HPG axis-related gene expression were determined to explore the endocrine disrupting effects of SEM acute exposure. In the brain, down-regulated transcription of follicle-stimulating hormone beta subunit (FHSβ), luteinizing hormone beta subunit (LHβ), and cytochrome P450 19B (CYP19B) in the 1 µg/LSEM was affected by reduced mRNA levels of seabream gonadotropin-releasing hormone (sGnRH). However, down-regulation of sGnRH was also observed after exposure to 10, 100, 1 000 µg/L SEM, while CYP19B and cGnRH mRNA levels were significantly up-regulated. In addition, the expressions of genes that involved in T and E2 synthesis, including steroidogenic protein, 3β-hydroxysteroid dehydrogenase, cytochrome P450 17, cytochrome P450 11A, were down-regulated in the gonads, while that of cytochrome P450 19A (CYP19A) was upregulated, which may explain the decrease in plasma sex hormones levels. Overall, our results show that SEM exerted potential endocrine disrupting effects by interfering with the HPG axis to inhibit the expression of steroidogenic protein and reduce the level of sex hormones in male zebrafish. |
| Key words: semicarbazide sex hormones hypothalamic-pituitary-gonadal axis endocrine disrupting effect zebrafish |
