| 引用本文: | 徐敏慧,颜成瑞,顾忠旗,黄继,李继姬,叶莹莹,夏立萍.厚壳贻贝(Mytilus coruscus)Hox基因家族的鉴定、进化与表达分析.海洋与湖沼,2023,3(3):895-906. |
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| 厚壳贻贝(Mytilus coruscus)Hox基因家族的鉴定、进化与表达分析 |
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徐敏慧1, 颜成瑞1, 顾忠旗2, 黄继2, 李继姬1, 叶莹莹1, 夏立萍1
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1.浙江海洋大学 国家海洋设施养殖工程技术研究中心 浙江 舟山 316022;2.嵊泗县海洋科技研究所 浙江 嵊泗 202450
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| 摘要: |
| 为探究厚壳贻贝Hox基因家族的定位、进化以及在不同成体组织中的表达模式, 基于厚壳贻贝全基因组数据, 结合生物信息学方法, 鉴定分析厚壳贻贝Hox基因家族成员的理化性质、染色体分布、系统进化关系、基因结构以及表达分析。结果显示, 从厚壳贻贝全基因组数据中鉴定出11条Hox基因序列, 并分别命名为Hox1-5、Antp、Lox2、Lox4、Lox5、Post1、Post2。对11条Hox序列的基本特征分析发现最长的是编码703个氨基酸的Hox2, 最短的是编码190个氨基酸Post2, 等电点5.11~10.22, 且不均等分布于同一条染色体上。亚细胞定位预测结果显示, 除Post1基因在细胞质和细胞核中均有发现, 其余10个基因亚细胞均定位在细胞核内。系统进化分析发现, 使用厚壳贻贝中鉴定出的101条氨基酸序列进行建树, 聚类的结果倾向于四类, 而从已鉴定的厚壳贻贝11个基因结合软体动物Hox建树结果分析, Post1和Post2聚为一支, Hox4、Antp、Lox5、Lox2和Lox4聚为一个大类, Hox1、Hox2和Hox3各自聚为一类Hox, 此外, Hox5单独聚为一类。厚壳贻贝不同组织的qRT-PCR结果显示,在鳃组织中Lox2、Lox4、Lox5的表达量远超其他基因的表达, 且这三个基因表达水平相差无几, 这可能与鳃组织的较快的新陈代谢速率有关。Hox4在性腺中的高表达, 推测可能与厚壳贻贝性腺发育有关。上述结果说明, Lox2、Lox4和Lox5可能与鳃上纤毛相关细胞的生长, 分化以及其他调控有关, Hox2、Hox3和Hox5以Hox4"为首"在外套膜中展现出高水平表达, 表明这四个Hox基因可能在贝壳形成方面具有重要作用。此结果为软体动物Hox基因家族功能提供新的研究视野, 同时对厚壳贻贝Hox基因的分类以及在成体中的调控和表达模式提出了新的证据。 |
| 关键词: Hox基因家族 基因鉴定 表达分析 |
| DOI：10.11693/hyhz20221000279 |
| 分类号:S917 |
| 基金项目:国家自然科学基金项目,42107301号；浙江省教育厅一般科研项目,Y202147628号 |
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| IDENTIFICATION, EVOLUTION, AND EXPRESSION PATTERN OF HOX FAMILY IN MYTILUS CORUSCUS |
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XU Min-Hui1, YAN Cheng-Rui1, GU Zhong-Qi2, HUANG Ji2, LI Ji-Ji1, YE Ying-Ying1, XIA Li-Ping1
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1.Zhejiang Ocean University, National Engineering Research Center for Marine Aquaculture, Zhoushan 316022, China;2.Shengsi Marine Science and Technology Institute, Shengsi 202450, China
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| Abstract: |
| To elucidate the localization, evolution, and expression patterns of the Hox gene (homeobox gene) family in different tissues of adult Mytilus coruscus, the physical and chemical properties, chromosomal distribution, phylogenetic relationships, gene structure, and expression of the gene family were analyzed with the whole genome data of M. coruscus and bioinformation methods. Eleven Hox sequences were identified from the whole mussel genome data, of which the longest encoded 703 amino acids, and the shortest one encoded 190 amino acids. The isoelectric points of the 11 Hox sequences ranged 5.11~10.22 and they distributed unequally on the same chromosome. Except for one gene that located in nucleus and cytoplasm, the remaining 10 genes are localized in the nucleus subcellularly. In addition, phylogenetic analysis on 101 amino acid sequences and the 11 Hox sequences of M. coruscus was conducted, 101 from which four general groups (clusters) could be recognized. The qRT-PCR results show that members of the family were expressed at different levels, from which we could infer that those members were related to specific functions, such as metabolism, gonad development, and shell formation. This study provided new insights on the function of the molluscan Hox gene family and presented new evidence on the classification of Hox genes of adult thick-shelled mussel as well as their regulation and expression patterns. |
| Key words: Mytilus coruscus Hox gene family gene identification expression analysis |
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