| 摘要: |
| 坛紫菜(于1988年采自青岛太平角海区人工养殖筏上)的水溶性色素粗提物经过硫酸铵沉淀和羟基磷灰石(HA)柱层析后,分离出藻蓝蛋白(RPC)、藻红蛋白(RPE)和变藻蓝蛋白(APC)。在中性介质中,其吸收光谱和荧光发射光谱与文献报道基本一致;但在酸性(pH=3)或碱性(pH=12)介质中,吸收光谱较在中性介质中有明显改变,原有的荧光性质也消失。RPC和APC只分离到一种聚集体,但RPE有两种不同的聚集体。用Sephadex G—100凝胶过滤方法测量藻胆蛋白的分子量分别为: RPC 117 000, APC 112 000,小分子RPE 38 000,大分子RPE 232 000。对三种藻胆蛋白的氨基酸分析的结果表明,三种藻胆蛋白中都是酸性氨基酸的含量大于碱性氨基酸的含量。 |
| 关键词: 坛紫菜 藻胆蛋白 化学组成 |
| DOI： |
| 分类号: |
| 基金项目:中国科学院海洋研究所调查研究报告第1865号。国家自然科学基金资助，3870137号 |
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| STUDIES ON CHEMICAL PROPERTIES AND COMPONENTS OF PHYCOBILIPROTEINS FROM PORPHYRA HAITANENSIS |
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Gao Hongfeng, Cao Wenda, Ji Minghou
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Institute of Oceanology, Academia Sinica, Qingdtto, 266071
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| Abstract: |
| The extract of soluble pigment of P. haitanensis harvested in Qingdao Bay in 1988 was precipitated by (NH4)2SO4 and then loaded on a hydroxylapatite column. Three types of phycobiliproteins, RPC, RPE and APC, were separated. The absorption spectra and room fluorescence emission of phycobiliproteins show no difference in neutual medium comparing with those reported formerly. but the absorption spectra in acidic or basic medium are more different from those in neutual medium, and fluorescence exhibiting in neutual medium disappeared.
Only one aggregate was separated from RPC and APC, but two aggregates were from RPE. The molecular weights of phycobiliproteins estimated on Sephadex G-100 column were: RPC 117 000, APC 122 000, small molecule RPE 38 000 and large molecule RPE 232000. There are some differences in absorption spectra, fluorescent emission and isoelectric points between two aggregates of RPE. The amino acid composition of phycobiliproteins indicated that Ala, Leu and Val are the dominate components in RPE and RPC, while LGU, Ile and Ala are the dominate components in APC. The content of acidic amino acids is hitgher than of basic ones. |
| Key words: Porphyra haitanensis, Phycobiliprotein, Chemical composition |
