摘要: |
于1989年10月-1990年9月分别先后在昌邑县下营镇对虾育苗场和青岛黄岛盐业公司养殖场采集中国对虾,运用光学、电子显微镜及组化方法,研究中国对虾卵母细胞的形态发育及其内部结构分化。结果表明,中国对虾卵母细胞的发育可分为4个主要时相:Ⅰ.早期分化时相,Ⅱ.滤泡初生期时相,Ⅲ.卵黄大量生成时相,Ⅳ.皮质棒生成时相。 |
关键词: 中国对虾 卵母细胞 发育 |
DOI: |
分类号: |
基金项目: |
|
A PRELIMINARY STUDY ON DEVELOPMENT OF OOCYTE OF PENAEUS CHINENSIS (OSBECK) |
Li Huaimei, Zhang Naiyu
|
Institute of Oceanology, Acadcmia Sinica, Qingdao 266071
|
Abstract: |
This paper describes the development phases of oocyte of cultured Penaeus chinensis. From October 1989 to September 1990, the cultured shrimps were caught in Xiaying Town Shrimp Hatchery, Changyi County and in Huangdao Salt Industry Corporation Shrimp Farm, Qingdao. Light and electron microscope and histochemical methods were used to study oocytes’ morphological changes and inner structure differentiations. Based on the size of the cell, the shape of the nuclei and nucleoli, the existence of the yolk granules, the emergence of cortical rods and the relationship between oocyte and follicle cells, the oocyte development was classified into 4 main phases, namely: I. early differentiation phase, II. folliculogenesis phase, III. vitellogenic phase, IV. cortical rods forming phase.
This study shows that: follicle cells probalbly supply substances needed for yolk formation; there are two resources, one from the oocyte itself and the other from the follicle cells, nucleoli shape change is one feature of during oocyte development, especially during early the differentiation phase of oocyte. |
Key words: Penaeus chinensis, Oocyte, Development |