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红藻真江篱质粒的发现
秦松1, 童顺1, 崔武2, 王希华1, 李亚非3, 吴光耀2, 张培军1, 曾呈奎1
1.中国科学院海洋研究所 青岛 266071;2.北京大学 北京 100871;3.中国人民解放军肝病研究所 北京 100700
摘要:
于1992年4月一1993年4月，用液氮研磨-苯酚／氯仿抽提-异丙醇沉淀的方法提取青岛沿海产紫菜、真江蓠、龙须菜、海膜4种红藻的总DNA；利用Hoechst－CsCl密度梯度起离心法将叶绿体DNA与核DNA分开。对抽取的叶绿体DNA梯度组分电泳结果表明，真江蓠样品出现质粒的电泳条带。以透射电镜观察到闭会环状DNA图像（周长平均值为4.3×10-7 m，约为1.3kb）；分子杂交结果显示，真江蓠质粒与其叶绿体DNA之间没有同源性，而与核DNA之间有一定同源性。
关键词:  红藻  真江蓠  质粒  CsCl密度离心
DOI：
分类号:
基金项目:中国科学院海洋研究所调查研究报告第2337号。中科院海洋研究所所长基金资助
PLASMID FROM THE RED ALGA GRACILARIA ASIATICA OF QINGDAO COAST IN CHINA
Qin Song1, Tong Shun1, Cui Wu2, Wang Xihua1, Li Yafei3, Wu Guangyao2, Zhang Peijun1, Zeng Chengkui ;(C.K. Tseng)1
1.Institute of Oceanology, Academia Sinica, Qingdao 266071;2.Department of Biology, Beijing, University, Beijing 100871;3.Institute for Liver Diseases, PLA, Beijing 100700
Abstract:
DNAs of the rhodophyte, Gracilaria asiatica, Gracilariopsis lemaneiformis, Porphyra sp. and Halymenia sp. from coast of Qingdao were characterized using Hoechst 33258-Cesium chloride ultracentrifugation from 1992 to 1993. Only in G. asiatica, an A-T rich plasmid was co-isolated while it co-migrated with chloroplast DNA in Hoechst-CsCl gradient. Circular and closed pattern was observed under an electron microscope. The girth was 4.3×10-7 m on average, and its size was ca. 1300bp. Both gel electrophresis and hybridization showed that its copy number was high. It has homologous sequence with nuclear DNA, instead of chloroplast DNA, which suggest that it may have integrated form.
Key words:  Rhodophyte, Graciloria asiatica, Plasmid, Cesium chloride gradien tultracentrifugation
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