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胁迫条件下盐藻β-胡萝卜素及其异构体累积的研究──盐度的影响
刘建国1, 赵学武2, 王玉君1, 王作芸2, 陈念洪1, 俞立东1, 吴以平2, 吴超元1
1.中国科学院海洋研究所 青岛 266071;2.青岛海洋大学生物系 青岛 266003
摘要:
于1989—1991年,在青岛室外以盐藻为实验材料,利用Johnson改良配方制作培养液,进行盐度(90-240)实验。结果表明,细胞生长的适宜盐度为120;当盐度超过120-240,盐度越高对细胞生长越不利,但越有利于细胞内β-胡萝卜素的累积;适宜于养殖盐藻生产β-胡萝卜素的盐度为240。研究证明,盐藻累积的β-胡萝卜素中至少含6种异构体,盐度增加不利于顺式异构体的累积。为生产防癌和延缓衰老等作用更强的顺式异构体,建议生产性养殖时适当降低盐度到210,以便累积更多的β-胡萝卜素及顺式异构体。
关键词:  盐藻  β-胡萝卜素  异构体  胁迫  累积
DOI:
分类号:
基金项目:中国科学院海洋研究所调查研究报告第2089号.国家自然科学基金资助,38970587号;中国科学院海洋研究所海洋生物学开放研究实验室资助,A20893005号
STUDY ON ACCUMULATION OF β-CAROTENE AND ITS ISOMERS IN DUNALIELLA SALINA UNDER ENVIRONMENTAL STRESS CONDITIONS — SALINITY EFFECT
Liu Jianguo1, Zhao Xuewu2, Wang Yujun1, Wang Zuoyun2, Chen Nianhong1, Yu Lidong1, Wu Yiping2, Wu Chaoyuan1
1.Institute of Oceanology, Academia Sinica, Qingdao 266071;2.Department of Biology, Ocean University of Qingdao, Qingdao 266003
Abstract:
The effect of salinity (S) on cell growth, accumulation of β-carotene and its isomers in Dunaliella salina Teodoresco strain 20 cultured outdoors in modified Johnson’s medium of salinity 90 - 240 was conducted in 1989 - 1991 in Qingdao. The results showed that the S favourable for growth is 120 and that for β-carotene accumulation is 240. The isomers of β-carotene in D. salina cultured in different S were analysed by HPLC with an Alox-T alumina column. Six isomers of β-carotene were found in different proportions dependent on the S of the culture medium. All trans β-carotene increased from 37.2% to 47.8% when the S increased from 90 to 240, but both 9-cis and 15-cis β-carotene decreased from 32.4% and 22.3% to 26.1% and 8.7% respectively. Decrease of S led to more total cis β-carotene accumulation. Because cis isomers of β-carotene are good anti-senscence substances and can also provent certain kinds of cancer. Proper lowering of the S is suggested for more cis β-carotene production in Dunaliella cultivation.
Key words:  Dunaliella salina, β-carotene, Isomer, Stress, Accumulation
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