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海湾扇贝肝素样多糖分离方法的研究
王长云, 管华诗, 林洪
青岛海洋大学海洋药物与食品研究所 青岛 266003
摘要:
于1992年1月-1993年2月，对从海湾扇贝中提取获得的糖胺聚糖（GAG）用乙醇和溴化十六烷基三甲铵（CTAB）分级分离，并对所得制品用琼脂糖凝胶电泳和高效液相色谱验证，以研究一种简便、快速的分离提纯方法。结果表明，扇贝GAG水溶液在乙醇量60％时分离获得的级分，其GAG－GTAB给合物再用2.0 mol/LNaCl溶液解离得到的主要级分，琼脂糖凝胶电泳和高效液相色谱均显示出单一区带，红外光谱类似干肝素的。证明这一提纯过程是一简便、快速的分离提纯方法。
关键词:  海湾扇贝  糖胺聚糖  分离提纯
DOI：
分类号:
基金项目:
FRACTIONATION OF HEPARINLIKE GLYCOSAMINOGLYCAN FROM BAY SCALLOP ARGOPECTEN IRRADIANS LARMARCK
Wang Changyun, Guan Huashi, Lin Hong
Institute of Marine Drugs and Foods, Occan University of Qingdao, Qingdao 266003
Abstract:
Glycosaminoglycans (GAG) isolated from Bay scallop (Argopecten irradians Larmarck) was dissolved and fractionated with ethanol. The main fraction obtained by precipitating with 60% ethanol was fractionated further with CTAB. The final fraction was isolated from the GAG—CTAB complex with 2.0 mol/L NaCl. Agarose gel electrophoresis and high performance liquid chromatography (HPLC) indicated that the final fraction contained single composition GAG whose infrared spectra showed that it was heparin-like. It is concluded that the process presented in this paper is a simple, convenient, and quick method for fractionation of GAG.
Key words:  Argopccten irradians Larmarck, Glycosaminoglycan, Fractionation
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