| 摘要: |
| 于1990，1993 - 1994年以捕自青岛近海的牙鲆亲鱼，采用冷休克法（0－4°C）进行多倍体的人工诱导实验，以期掌握牙鲆细胞遗传学特征和研究遗传育种方法。通过实验得到了三倍体牙鲆并进行培育。在原肠胚期采用空气干燥法、Giemsa染色获得正常二倍体、三倍体的染色体制片。镜检得，牙鲆二倍体染色体以2n=48，均为端部着丝点染色体，核型为24t，其管数NF=48；三倍体的染色体3n=72，也都为端部着丝点染色体，其臂数NF=72，并依据二倍体的枝型得出了三倍体的核型图。 |
| 关键词: 牙鲆 二倍体 三倍体 核型 |
| DOI： |
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| 基金项目:山东省科委资助!983120－1 |
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| KARYOTYPE EVIDENCES OF TRIPLO,IDY IN THE LEFT-EYED FLOUNDER, PARALICHTHYS OLIVACEUS (T. & S.) |
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You Feng, Liu Jing
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Institute of Oceanology, Academia Sinica, Qingdao 266071
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| Abstract: |
| Studies on triploidy induction in the left-eyed flounder, Paralichzhys alivaceur (T. & S.) from the coast of Qingdao were carried out using cold-shock technique (0 - 4°C) in 1990, 1993 and 1994 in Qingdao. The triploid left-eyed flounder were obtained and cultured under normal conditions. This paper presents karyotype evidences of induced triploidy and diploid left-eyed flounder. Samples for chromosome studies of diploidy and induced triploidy were prepared according to the routine air-dried method and stained with a Giemsa solution. Observation under microscope reveals that there are 48 chromosomes in diploidy which are acrocentric chromosomes and its karyotype formula is 24t, NF = 48, while in triploidy there are 72 chromosomes which are also acrocentric chromosomes and its arm number NF= 72.
The different chromosome numbers of diploidy in the left-eyed flounder obtained in this study were different from those of Nogosu (1960) and Sakamoto et al. (1980). We consider that there might be polymorphism in karyotypes of the left-eyed flounder due to the different geological populations. This study also showed that the early stage of triploidy in the left-eyed flounder could be identified very well in the gastrula stage and that its triploid karyotype is made on the basis of the diploid karyotype and the resources of chromosomes. |
| Key words: Paralichthys olivaceus (T. & S.), Diplody, Triploidy, Karyotype |
