| 摘要: |
| 建立了顶空GC/FPD测定海水中二甲基硫（DMS）和浮游植物细胞中二甲基硫丙酸（DMSP）的方法，并研究盐度、温度、气液相比DMS诸因素对DMS项空灵敏度的影响。该法对DMS测定的相对标准偏差均小于6％，平均回收率为106％，最低检出限为20ng/L。细胞DMSP先经碱作用转化为DMS，在50°C下作用时间不少于6h，峰高与浓度的双对数线性相关系数大于0.99。对1994年冬、1995年夏采自胶州湾的实际样品分析结果表明，本法可用于一般海洋样品的测定。 |
| 关键词: 二甲基硫 二甲基硫丙酸 顶空气相色谱 海水 浮游植物细胞 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金!49476298;中国科学院院长基金 |
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| DETERMINATION OF DIMETHYLSULFIDE IN SEAWATER AND DIMETHYLSULPHONIOPROPIONATE IN PHYTOPLANKTON CELLS BY HEADSPACE GC/FPD METHOD |
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Wang Yonghua1, Jiao Nianzhi2
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1.Department of Geogrophy, Beijing University, Beijing 100871;2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
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| Abstract: |
| This paper presents a modified Headspace GC/FPD method for determination of dimethylsulfide (DMS) in seawater and dimethylsulphoniopropionate
(DMSP) in phytoplankton cells. The effects of temperature, gas/liquid phase ratio, and salt concentration on headspace sensitivity were investigated. The optimum condition of these three factors are at 40°C, 0.5% and 20% respectively. The method ’s detection limit is 20 ng/L. The relative standard deviation is less than 6%, the average recovery rate is 106%; relative error is less than 10%. The double logarithm linear correlation cient between peak height and DMS concentrations is better than 0.99. The completion time of DMS release from cell DMSP under alkaline condition (10 mol/L NaOH) is at least 6 hours. This procedure can be used for the measurement of DMS and DMSP in a variety of natural samples. |
| Key words: Dimethylsulfide (DMS), Dimethylsulphoniopropionate (DMSP), Headspace, Gas chromatograph, Seawater, Phytoplankton cell |
