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钝顶螺旋藻的硝酸还原酶与氢酶之间的电子竞争
张慧苗, 顾天青
中国科学院植物研究所 北京 100044
摘要:
于1994年6－11月，运用生物化学方法以钝项螺旋藻为材料制备整体细胞和无细胞提取物。测定表明，整体细胞硝酸还原酶与氢酶活性的相互关系和无细胞提取物氢酶与硝酸还原酶的（对还原甲基紫精）Km值，分别为2.299×10-7 mol/L和1.098×10-7 mol/L；证实在硝酸还原酶与氢酶之间存在着电子竞争。
关键词:  钝顶螺旋藻  硝酸还原酶  氢酶  电子竞争
DOI：
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基金项目:国家自然科学基金资助项目，3880189号
THE ELECTRON COMPETITION BETWEEN NITRATE REDUCTASE AND HYDROGENASE IN SPIRULINA PLATENSIS
Zhang Huimiao, Gu Tianqing
Institute of Botany, Chinese Academy of Siences, Beijing 100044
Abstract:
The intact cell and cell-free extracts of Spirulina platensis for this June to November, 1994 were crushed in this study showing that nitrate reductase and hydrogenase activities were 1.0 nmol/(h*mg) and 55.0 nmol/(h*mg) in NO3- culture medium, respectively and that Km of cell-free nitrate reductase and hydrogenase (methylviologen in reduced state) were 1.098×10-7 mol/L and 2.299 ×10-7 mol/L, respectively. The results above confirm electron competition exists between nitrate reductase and hydrogenase in Spirulina platensis.
Key words:  Spirulina platensis, Nitrate reductase, Hydrogenase, Electron competition
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