| 摘要: |
| 于1995年11月在山东荣城大鱼岛鲍鱼养殖厂采集皱纹盘鲍，以大肠杆菌、弧菌为诱导物，将鲍分为三组分别进行注射，在注射后的5，18，32，50h分别取鲍血淋巴，测定其抗菌、溶菌、酚氧化酶和超氧化物歧化酶活力，并研究注射组各项活力的变化规律。实验结果表明，适当的刺激可增加皱纹盘鲍的抗病能力。 |
| 关键词: 皱纹盘鲍 血淋巴 溶菌 抗菌 酚氧化酚 超氧化物 歧化酶活力 |
| DOI： |
| 分类号: |
| 基金项目:国家攀登计划B“海洋生物优抗研究”项目资助基金，PD-BO-3 |
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| STUDY ON THE CHANGES OF SOME FACTORS IN HAEMOLYMPH OF INDUCED HALIOHS DISCUS HANNAI |
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Ding Xiuyun1, Li Guangyou2, Zhai Yumei1
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1.Department of Bioloyg,Shandong University, Jinan 250100;2.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071
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| Abstract: |
| Speciments of Haliotis discus Hannai were collected from Rongcheng, Shandong Province in November 1995. The levels of antibacterial, acteriolytic, phenoloxidase and superoxide dismutase (SOD) activities and the blood cell number in the haemolymph of Haliotis discus Hannai were tested at 5, 18, 32, 50h after injection with filtered seawater (controlled group), with E. coli, and with Vibrio sp. The results showed that those infected with E. coli had significant elevations in bacteriolytic activity in the serum at 32h after injection: those infected with Vrbrio sp. had the highest level at 5h after injection. This proved that the two kinds of bacteria could induce elevations in bacteriolytic activity in the haemolymph of Haliotis discus Hannai. It is suggested that infection with Vibrio sp. and E. coli could induce elevations in antibacterial activity and blood cell number, but that the effect of infection with the two kinds of bacteria on phenoloxidase and SOD activities was not obvious. All these proved that proper stimulus can increase the immune protency of H. discus Hannai. |
| Key words: Haliotis discus Hannai, Haemolymph, antibacterial, Bacteriolytic, Phenoloxidase, SOD activity |
