| 摘要: |
| 于1993年8月，从山东省昌邑县下营镇患败血病的中国对虾血淋巴内分离到多株细菌，其中2株人工感染证实为病原菌;经50多项形态、生理、生化特性测试鉴定为产气弧菌门Vibriogazogenes,其主要特性为:革兰氏阴性，弧状，极生单鞭毛，产生红色素，氧化酶呈阳性，精氨酸双水解酶、赖氨酸脱羧酶、鸟氨酸脱羧酶均为阴性，淀粉酶、明胶酶为阳性，硝酸盐还原阴性，利用木糖、水杨苷、山梨醇发酵葡萄糖产气，利用柠檬酸盐为阳性。生长最适温度在25-30℃，最适NaCl在1.0％-2.0％（W／V），最适pH在7.5-8.5。对柱晶白霉素、先锋霉素5号、新生霉素、四环素、活霉素、羧苄青霉素、麦迪霉素等极为敏感。产气弧菌引起中国对虾败血病在国内外属首次报道。 |
| 关键词: 中国对虾 产气弧菌 败血病 |
| DOI： |
| 分类号: |
| 基金项目:国家“八五”应急攻关项目，85-21-03-02号 |
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| VIBRIO GAZOGENES, A NEW VIBRIO PATHOGEN FROM SEPTICEMIA OF PENAEUS CHINENSIS |
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Zhan Wenbin, Zhou Li, Yu Kaikang, Chen Zhangqun, Meng Qingxian
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College of Fisheries,Ocean University of Qingdao,Qingdao 266003
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| Abstract: |
| After cultivation at 25 °C in 2216E agar medium, two strains of Vibrio gazogenes were isolated from the blood of shrimp affected by epizootic septicemia. The shrimp, Penaeus chinensis, were collected from Xiaying Town, Changyi County, Shandong Province in August 1993. Artificial infections by injection, dipping and feeding methods proved that these isolates were some of the pathogens of the disease. The isolates were tested for more than 50 unit characters and according to Bergey’s Manual of Systematic Bacteriology 9th, were classified as Vibrio gazogenes.
The bacteria were gram negative, curved rods, with single polar flagellum, produced red pigment. Oxidase, arginine dihydrolase, lysine and ornithine decarboxylase tests were negative. Some of the other characteristics, such as production of gas from glucose, utilization of xylose, salicin and sorbitol, negative for nitrates reduced to nitrites, citrate utilization, positive for amylase, gelatinase and lipase production, sensitivity to O / 129, were also proved.
Optimum growth temperature, pH and salinity were 25°C to 30°C, 7.5 to 8.5 and 1.0% to 20% sodium chloride respectively.
These isolates were highly sensitive to Leucomycin, Cefazolin, Novobiocin, Tetracycline, Carbenicillin, Lincomycini and Medemycin. This is the first reported of V. gazogenes as a pathogen of shrimp disease. |
| Key words: Penaeus chinensis, Vibrio gazogenes, Septicemia |
