| 摘要: |
| 于1997年4月在借鉴国外资料基础上，组建起了一种适用于实验室培养光自养生物（如光合细菌、微藻和单克隆无性繁殖系）的气升式双螺旋管道串联的光生物反应器。该装置内可插入pH值电极、溶解氧电极、温度电极、细胞密度电极、粘度电极等，通过传感器获得的电信号，进一步传导入微机，实现按程序化自动记录和控制有关参数，进行一次性、间歇和连续悬浮培养。以红球藻为材料进行培养的初步结果表明，培养时间较常规方法缩短了一半，而细胞数量增加了3倍，细胞内的虾青素基本没有变化，即通过该技术可使生物量或有效成分产量提高6倍。 |
| 关键词: 管道式光生物反应器 生物技术 微藻 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39500114号 |
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| INSTALLATION OF A TWO-STAGE AIR-LIFT PHOTOBIOREACTOR |
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LIU Jian-guo,ZHANG Jing-pu,YIN Ming-yan,MENG Zhao-cai
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Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071
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| Abstract: |
| A 10-L two-stage air-lift helical tubular photobioreactor has been installed in our laboratory with transparent plastic tubes coiled around a metal net. The coiled bioreactor is connected by recirculated loops with two sets of gas exchangers, heat exchangers and pumps. The bioreactor can be illuminated by 8 day light fluorescent lamps mounted within the cylinder-like system, providing a light intensity ranging between 0 and 250 μE/(m2s). However, strong illumination can be obtained also from sunshine if it is located near the window or outdoor. This laboratory scale photobioreactor with a high surface-to-volume ratio (about 200 m-1) is particularly suitable for culturing microalgae, phototrophic bacteria and other phototrophic microorganism in batch, semi-continuous and continuous modes. Preliminary results show that both biomass of Haematococcus pluvialis and astaxanthin production increased 6-fold compared with the culture in flask. |
| Key words: Tubular photobioreactor, Biotechnology, Microalgae |
