| 摘要: |
| 采用聚合酶键扩增反应（PCR）和限制性片段长度多态性分析（RFLP）方法，对1992年5月和1994年间月采自中国南海大鹏湾、大亚湾、香港海域的赤潮有毒甲藻塔玛亚历山大藻8个不同地理株的核糖体小亚基RNA基因（Ss－rDNA）进行分析，并与北美、西欧等地的比较。结果表明，中国南海的塔玛亚历山大藻缺少B基因，这与北美等地的塔玛亚历山大藻（包括有毒和无毒株）不同，而与西欧、澳洲等地的有毒和无毒株相似，提示B基因的存在可以作为一种分子地理标记而不是藻种有毒的标记。 |
| 关键词: 塔玛亚历山大藻 分子地理标记 核糖体小亚基RNA基因 限制性片段 赤潮 |
| DOI： |
| 分类号: |
| 基金项目:“九五”国家自然科学基金资助项目，39790110号 |
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| ANALYSIS OF MOLECULAR BIOGEOGRAPHIC MARKER ON RED TIDE TOXIC ALEXANDRIUM TAMARENSE IN THE SOUTY CHINA SEA |
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CHEN Yue-qin1, QIU Xiao-zhong1, QU Liang-hu1, ZENG Long-mei2, QI Yu-zao3, ZHENG Lei3
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1.School of Life Sciences,School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;2.School of Chemistry and Chemical Technology, Zhongshan University,Guangzhou,510275;3.Institute of Hydrobiology,Jinan University,guanzhou,510632
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| Abstract: |
| In this study, cultures of eight strains of red tide toxic Alexandrium tamarense were established from germinated cysts or vegetative cells isolated from Dapeng Bay, Daya Bay and the Hong Kong area in May 1992 and December 1994, and a method of PCR amplification with individual cells was used to amplify the small subunit ribosomal RNA genes (Ss-rDNA) of eight strains for a restriction fragment length polymorphism (RFLP) assay. PCR-RFLP analysis of Ss-rDNA on the red tide toxic A. tamarense demonstrated that the strains of A. tamarense in the South China Sea have Ss-rDNA A gene and lack of B gene, which was the same with that of the strains in western Japan and Australia (toxic or non-toxic), but different from that of the strains in North American seas. The genetic consistence of toxic A. tamarense in China seas suggested that the increase in the geographic range of A. tamarense in this sea area has possibly a local origin. In addition, it was demonstrated that the type of Ss-rDNA could be as a report marker for the identification and monitoring of the origin and dispersal of toxic species of Alexandrium. |
| Key words: Alexandrium tamarense, Molecular biogeographic marker, small subunit ribosomal, RNA genes (Ss－rDNA), RFLP, Red tide |
