摘要: |
于1996年4–5月,在山东即墨市田横镇对虾育苗场,以包膜维生素C和维生素C磷酸酯镁两种类型的维生素C添加饵料饲喂中国对虾状幼体和糠虾幼体,以便研究在饵料中添加不同类型的维生素C对中国对虾早期幼体生长与存活的影响以及适宜的添加量。结果表明,添加包膜维生素C饲喂状幼体的效果优于维生素C磷酸酯镁,其最适添加量为2mg/g饵料;添加维生素C磷酸酯镁饲喂糠虾幼体的效果优于包膜维生素C,添加量为1—5mg/g饵料时,对幼体的影响不显著。 |
关键词: 中国对虾 包膜维生素C 维生素C磷酸酯镁 适宜添加量 |
DOI: |
分类号: |
基金项目:国家攀登计划B资助项目,PDB-6-2-2号 |
|
EFFECTS OF COATED ASCORBIC ACID AND MAGNESIUM ASCORBYL-2-PHOSPHATE IN THE DIET ON THE LARVAE OF SHRIMP PENAEUS CHINENSIS |
MA Shen, ZHANG Dao-bo, WANG Ke-xing
|
Fishery College, Ocean University of Qingdao, Qingdao, 266003
|
Abstract: |
The formulated feeds added with coated ascorbic acid and magnesium ascorbyl-2-phosphate at five contents were used to feed the larvae of shrimp Penaeus chinensis to determine the effects of coated ascorbic acid and magnesium ascorbyl-2-phosphate on the larvae and the optimum quantification of ascorbic acid in the diet.
The larvae used in the experiment were hatched and cultured from the broodstock captured from the Yellow Sea, and nursed in captivity. The feeds were made from headless shell-off shrimp, chicken egg, and bean curd; sodium alginate was added as binder. The ascorbic acid contents of coated ascorbic acid and magnesium ascorbyl-2-phosphate were 92% and 46%, respectively, and the final contents in the diet were 1, 2, 3, 4 and 5mg for every g diet. All the raw materials were homogenized by blender and mixed with sodium alginate, and then coated ascorbic acid and magnesium ascorbyl-2-phosphate were added by the given content. After being baked at 60°C, ground and sieved, the feeds were finally prepared by coating with cyclohexane saturated cholesterol and sieved again. Larvae were cultured at density of 33.33 ind/L in plastic buckets, each of which contained 6 liters sand filtered seawater. During the experiment, the water was aerated and changed at the rate of 0.5 liter per day. Other nursing conditions were temperature at 23–24°C, salinity at 33.12, pH at 7.9–8.2 for zoea larvae; and temperature at 24–25°C, salinity at 34.72, pH at 8.0–8.2 for mysis larvae. The larvae were fed with the feeds prepared at a total amount of 16–20 mg/L each day.
The results indicate that the coated ascorbic acid functioned better than magnesium ascorbyl-2-phosphate to zoea larvae and the optimum doze was 2 mg/g diet, while the magnesium ascorbyl-2-phosphate did better than the coated ascorbic acid in the mysis stages and the doze used in the test showed no significant difference. |
Key words: Penaeus chinensis, Coated ascorbic acid, Magnesium ascorbyl-2-phosphate, Optimum quantification in diet |