| 摘要: |
| 根据1995年9月利用日本《神鹰丸》号调查船在黄海32°00'–35°00'N，122°00'–127°00'E海区的19个站位上进行的中日联合调查研究中叶绿素a含量的调查资料,探讨南黄海海区夏末初叶绿素a含量的变化、平面分布、断面分布和垂直分布状况,以及与海域环境因子之间的关系。在每个测站上作垂直取样,表层水用圆塑料桶取自海表面,深层水用日本提供的专用采水器采集,取出水样立即量取200ml,用玻璃纤维滤膜过滤浓缩,并加入2％的饱和碳酸镁溶液,防止叶绿素脱镁,然后保存在冰箱内（-1°C）,用冰桶带回实验室进行分析。将载有浮游植物的滤膜放入闪烁瓶内加入10ml 90％的丙酮溶液,在冰箱内提取24h。用萃取荧光法测定叶绿素a含量。结果表明,该海域的叶绿素a含量较高,平均值为1.14mg／m3,其变化范围为0.10–7.76mg／m3，最高值在次表层。（1）平面分布:各层次平面分布特征差异较大。33°00'–33°30'N之间叶绿素a含量均较低，低于0.50mg／m3。33°30'N以北，叶绿素a含量低于0.20mg／m3，而33°00'N以南，除济州岛附近的17–19导站以外，叶绿素a含量均较高，高于100mg／m3。（2）断面分布:水深在30m时，叶绿素a含量的高值区在20m以上水体的次表层中，而水深为50–80m时，其高值分布在20–40m的次表层中。（3）叶绿素a的垂直分布也体现了断面分布的特征。所以作者认为，光是浮游植物生长和繁殖的重要因子之一。 |
| 关键词: 叶绿素a 南黄海 分布特征 生态学 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，49546004号 |
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| THE DISTRIBUTION CHARACTERISTICS OF CHLOROPHYLL-a IN LATER SUMMER IN THE SOUTHERN YELLOW SEA AREA |
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Li Bao-hua1, FU Ke-cun1, ZENG Xiao-qi2
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1.First Institute of Oceanography, Stat Oceanic Administration, Qingdao, 266003;2.Department of Chemistry, Ocean University of Qingdao, 266003
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| Abstract: |
| Based in the chlorophyll-a data obtained from samples collected from 19 stations during “the Research Vessel Shenyou Maru cruise in the Southern Yellow Sea area” (32°00'–35°00'N, 122°00'–127°00'E) from 1 to 7 September 1995, this paper expounds in detail the situations of the horizontal, sectional and vertical distributions of the chlorophyll-a as well as the relationships between chlorophyll-a variations and environmental factors. The extractive fluorimetry was used for pigments determination. Vertical sampling was carried out at each station, water samples being taken from the depths of 0m, 5m, 10m, 15m, 20m, 30m, 50 and 80m. Fluorometric determinations of chlorophyll-a concentration were made with a Turner Design model 10 fluorometer. 200ml of the water sample were filtered with a micro-pore filter membrane immediately, stored in the refrigerator (-1°C), and brought back to the laboratory with an ice bucket for analysis. This filter membranes which were fully loaded with phytoplankton samples were put into scintillation bottles, and after addition of 90% acetone solution preserved in a refrigerator for extraction for 24h, and then taken out for the determination at room temperature, immediately. Calculation of chlorophyll-a (Chl-a):
Chl-a (mg/m3)=[F-(Fa-Fb)υ]/VS
where F is the correctional factor; Fa, the absorption value before acidification; Fb, the absorption value after acidification; υ, the volume of the extraction; V, the volume of the to be filtered solution; S, the light regulation value.
Analysis of the experimental results are as follows. The concentrations of chlorophyll-a were all high in the investigated area, with an average value of 1.14mg/m3, the variation ranged from 0.10 to 7.76 mg/m3. The highest values were in the sub-surface water. The horizontal distribution of chlorophyll-a reflected the complicated variations in the abundance and distribution of phytoplankton (Fig.2). The horizontal distribution of chlorophyll-a were different in the various layers. It is worth taking notice of the existence of a high chlorophyll-a concentration in the southern part of the area (33°00'N) except stations 17–19 near Jizhou Island. The concentrations there were all higher than 1.00 mg/m3, but low in the northern part of the area (33°30'N), lower than 0.20 mg/m3. For the cross-sectional distributions (Fig.3), when the water was 30m deep, the chlorophyll-a was higher in the water body above 20m; when the depth was 80m, the subsurface chlorophyll-a maximum value existed from 20–40m. with regard to the vertical distributions (Fig.4), chlorophyll-a has a layered structure. The characteristics of vertical distribution of chlorophyll-a show the same as those of the sectional distributions of chlorophyll-a in the southern Yellow Sea area. At the same time this paper also discussed the topic that light is one of the important factors affecting the growth and multiplication of phytoplankton. |
| Key words: Chlorophyll-a, Southern Yellow Sea, Distribution characteristics, Ecology |
