| 摘要: |
| 于1993年6月在大连太平洋海珍品养殖公司的12个采集点分离到河流弧菌Ⅱ的噬菌体，依据过量噬菌体可以全部裂解其宿主菌河流弧菌Ⅱ的特性，用噬菌体对皱纹盘鲍脓疱病进行了生物防治研究。结果表明，使用一定浓度的噬菌体可以有效地治疗或推迟脓疱病引起的鲍死亡，可将鲍的成活率提高50％以上。探讨了在噬菌体生物防治中遇到的难题及解决办法、噬菌体分离规律等问题。 |
| 关键词: 皱纹盘鲍 脓疱病 噬菌体 生物防治 |
| DOI： |
| 分类号: |
| 基金项目:大连市科学技术委员会资助项目，[1995]42号 |
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| STUDIES ON BACTERIOPHAGE CONTROL PUSTULE DISEASE OF ABALONE HALIOTIS DISCUS HANNAI |
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LI Tai-wu1, XIANG Jian-hai1, LIU Rui-yu1, DING Ming-jin2, SHI ping2, WANG Shi-hong2
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1.Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071;2.Aquacultural Company of Dalian, Dalian, 116023
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| Abstract: |
| The isolation, purification and propagation of bacteriophage of Vibrio fluvialis- II were studied. The phage was isolated from 12 different water samples, using the normal isolation procedure. The Vibrio fluvialis- II can grow well on STA (seawater, tryptone and agar) medium, which was used as a host and test culture.
The plaques were obtained by the agar bilayer method. Concentrated phage suspensions were obtained from plates by washing them with 2% NaCl solution (e.g. add 4 ml 2% NaCl solution into each plate); they were then put into refrigerator at 4 °C overnight. The phage suspensions from the plate were added into a 5 ml tube. The host bacteria were removed by centrifugation at 8000 r/min and filter, and were purified and propagated by picking the single plaque repeatedly.
The bacteria Vibrio fluvialis- II can be all split using the phage with a high concentration. The effect of bacteriophage control pustule disease of abalone by muscular injection and infection on the wound in abalone foot can raise the survival rate of the abalone by up to 50%.
Electron microscopic examination of the material taken from the plaques of the phage show that they contained simultaneously two forms of phage particles, with large and small heads, while their tails were morphologically identical. Numerous successive passages of the material taken from single plaque did not make it possible to separate small and large phage particles. |
| Key words: Haliotis discus hannai, Pustule disease, Bacteriophage, Biological control |
