| 摘要: |
| 于1997年11月，在北黄海采用现场实验的方法，研究营养盐对浮游植物的上行效应。将浮游植物分为3个粒级：网采浮游植物（netphytoplankton，简称net-，20－200μm）、微型浮植物（nanophytoplankton，简称nano-，2—20μm）和超微型浮游植物（picophytoplankton，简称pico-，＜2μm）。实验期间Pico-叶绿素a的生物量占整个浮游植物群落总叶绿素a生物量的50％以上，net-和nano-贡献了总叶绿素a生物量的其余部分。结果表明，无论硝酸盐还是磷酸盐的添加，与0时刻相比较，实验组net-叶绿素a生物量都增加。实验结束时刻各组叶绿素a生物量的结果比较显示，低N组各粒级浮游植物叶绿素a的生物量与对照组相比都无显著差异（p＜0.05）。高N组net-叶绿素a的生物量与对照组相比有显著差异（p＜0.05）。低P组net-叶绿素a的生物量与对照组相比有显著差异（p＜0.05）；但nano-和pico-叶绿素a生物量与对照组相比均无显著差异（p＜005）。高P组各粒级浮游植物叶绿素a生物量与对照组相比都有显著差异（p＜0.05）。在这一季节，黄海可能同时存在的N、P营养盐的限制作用。 |
| 关键词: 浮游植物 营养盐 限制性 黄海 |
| DOI： |
| 分类号: |
| 基金项目:国家自然科学重点基金资助项目，39630O60号;国家自然科学基金资助项目，39625008号 |
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| THE PRIMARY RESEARCH OF NUTRIENT LIMITATION OF PHYTOPLANKTON IN NORTHERN YELLOW SEA |
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WANG Yong,JIAO Nian-zhi
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Institute of Oceanology, The Chinese Academy of Sciences,Qingdao, 266071
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| Abstract: |
| During a cruise to the northern Yellow Sea on board the R/V “Science I” in November, 1997, surface seawater was sampled and incubated in-situ in order to study nutrient limitation of phytoplankton growth. Two experiments were performed at different stations, one with addition of nitrate, and the other with addition of phosphate. Phytoplankton was divided into three size fractions: netphytoplankton (20－200μm), nanophytoplankton (2—20μm) and picophytoplankton (＜2μm). Picophytoplankton contributed more than 50% chlorophyll-a biomass to total phytoplankton community chlorophyll-a biomass during the experimental period, netphytoplankton and nanophytoplankton contributed others. The concentration of dissolved inorganic nitrogen and dissolved inorganic phosphorus in surface seawater were low in the first experiment but high in the second experiment. The ratios of N:P were changed from 14.2 in the first experiment to 19.4 in the second experiment. Two in-situ incubation results indicated that phytoplankton experienced nutrient limitation during this season. The chlorophyll-a results of bioassays showed significant stimulation of netphytoplankton with the addition of phosphate or nitrate xomparing to onset of each experiment. The results of chlorophyll-a of nanophytoplankton and picophytoplankton at 72 h showed there was no significant difference between control carboys and nitrate addition carboys (p>0.05). Netphytoplankton chlorophyll-a were significantly different between control carboys and phosphate addition carboys; nanophytoplankton and picophytoplankton chlorophyll-a were not significantly different between control carboys and low concentration phosphate addition carboys, but significant different between control carboys and high concentration phosphate addition carboys (p<0.05). |
| Key words: Phytoplankton, Nutrient, Limitation, Yellow Sea |
