| 摘要: |
| 于1996年6月–1997年6月，在山东青岛、日照沿海采集日本鬼鲉，采用光学显微镜和电子显微镜技术观察其背鳍棘中毒腺的形态结构，研究其致毒机理。结果表明，日本鬼鲉背鳍的毒腺呈梭形，位于背鳍棘背外侧的两纵沟内，有结缔组织包绕。在毒腺内发现两种类型的细胞：Ⅰ型细胞的胞质为均质状，单核；Ⅱ型细胞胞质内充满颗粒，未见细胞核。两种毒腺细胞均呈多边棱柱体，呈辐射状排列。Ⅰ型细胞胞质电子密度中等，有均匀分布的细小颗粒，细胞内仅见少而散在分布的线粒体和线头状结构；Ⅱ型细胞颗粒为许多囊泡及电子密度不同的颗粒状物质。腺体内下方的组织内有一种小细胞，其胞质内含有溶酶体样结构，其内涵物与Ⅰ型细胞胞质类似。提示该毒腺的细胞不同于哺乳动物的分泌细胞。 |
| 关键词: 日本鬼鮋 毒腺 结构 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目，39270645号;山东省教育委员会资助项目，JglK01号 |
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| THE TOXICITY RESEARCH OF SPINE POISONING FISH IN CHINA COASTAL WATERS—THE STRUCTURE OF VENOM GLAND IN DORSAL SPINE OF INIMICUS JAPONICUS |
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LIU Xiao-ping, YU Ye-jun, ZHANG Ke-ling
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Department of Histology and Embryology, Medical College of Qingdao University, Qingdao, 266021
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| Abstract: |
| In this paper the structure of venom gland in dorsal spine of Inimicus japonicus was studied by means of light microscopy, transmission and scanning electromicroscopy. The glands located in either side grooves in dorsal spine were covered with a fibrous capsule. There are two types of cells in the venom glands. Electromicroscopy showed that both types were arranged in a radiation pattern. The cytoplasm of type I cells appeared to be homogeneous with electron-dense material. The tubular cisterns formed by invagination of cell membrane, mitochondria and threadlike structure were observed. However, no rough endoplasmic reticulum, Golgi complex, smooth endoplasmic reticulum and lipid droplet were found in type I cells. Type II cells were filled with different electron-dense granules, which were in monomer or polymer form. There were many dilated cisterns near the cytoplasm and large electron-dense ball-like structures without covering membrane among the granules. The type II cells were disrupted easily. There were some small cells in the connective tissues at the inner and infra parts of the venom gland. There were lysosome-like structure in the small cells whose contents were similar to the cytoplasm of type I cells. The results suggest that cells of venom gland appear to be unique in comparison with the secretory cells of mammal. Type I cells may be without the ability of synthesizing protein and may be derived from the small cells. |
| Key words: Inimicus japonicus, Venom gland, Structure |
