| 摘要: |
| 于1996–1997年春、秋渔汛季节，在山东青岛、日照沿海采集日本鬼鲉，分离毒腺，提取毒腺粗提液，对大鼠后肢进行肌肉注射，采用组织学、组织化学、放射免疫学方法观察和检测局部皮肤和肌肉的组织结构改变、注射局部肌细胞内的琥珀酸脱氢酶（SDH）及神经肌肉连接处的已酰胆碱酯酶（AchE）活性变化以及神经肽──P物质和生长抑素含量的改变。结果表明，毒腺粗提液局部注射可造成大鼠肢体麻痹；局部皮肤和肌肉组织明显肿胀，肌细胞有损伤，其内SDH活性显著降低，肌细胞及其间质水肿、血管扩张、伴有出血现象；神经肌肉连接处AchE活性显著增强；局部肌肉组织中P物质含量显著升高，而生长抑素含量显著降低。提示毒棘螫伤造成的剧烈疼痛和肿胀可能与P物质有关，造成肢体麻痹可能与肌细胞损伤及神经肌肉接头处乙酰胆碱分解过程增强有关。 |
| 关键词: 日本鬼鲉 乙酸胆碱酯酶 P物质 生长抑素 |
| DOI：10.11693/hyhz200002003003 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39270645号;山东省教育委员会资助项目，J91K01号 |
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| TOXICITY RESEARCH OF SPINE POISONING FISH FREQUENTLY FOUND IN CHINA COASTAL WATERS ──EFFECTS OF INJECTION WITH CRUDE VENOM EXTRACTION OF STONEFISH (INIMICUS JAPONICUS) ON THE STRUCTURE AND METABOLISM IN THE RAT WOUND TISSUES |
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LIU Xiao-ping, ZHANG Ke-ling, YU Ye-jun
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Medical College of Qingdao University, Qingdao, 266021
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| Abstract: |
| In order to study the mechanism of venomous effects of Inimicus japonicus, in this paper, we used Histologic, Histochemical techniques and Immunoradioassay (RIA) to study the venomous effects on the structure and the metabolisms of Acetylcholinesterase (AchE) and Succinate Dehydrogenase(SDH), and on the contents of Substance P (SP) and Somatostatin (SS) in the wound tissues. The results show that the injection of the crude venom from Inimicus japonicus could cause the wound tissues severe swelling, damage and hemorrhage, decreased the SDH activities, specially in the red and intermediate muscle cells, but markedly increased the AchE activities and the contents of SP. This indicates that the cause of severe pain and swelling stung by Inimicus japonicus may be related to SP and the cause of paresthesia may be mediated by accelerating the degradation of Ach, muscular and nervous fiber damage induced by crude venom. |
| Key words: Stonefish (Inimicus Japonicus), AchE, SP, SS |
