| 摘要: |
| 1997年6月在山东省日照市岚山头附近海域收集日本鬼鲉,采用放射免疫学方法检测了注射毒腺粗提物大鼠局部肌肉组织、血浆、脊髓、延髓、中脑、下丘脑及免疫器官内P物质和生长抑素含量变化;用MTT法检测了毒腺粗提物在整体和离体条件下对免疫细胞活性的影响;用生化方法检测了毒腺组织中透明质酸酶活性。结果表明,与对照组大鼠相比,实验组大鼠局部肌肉及延髓、中脑、胸腺和脾脏中P物质含量增加,而下丘脑中P物质含量下降。生长抑素在中脑、延髓、脊髓、胸腺中显著增高。毒腺粗提物对胸腺细胞和脾细胞的活性具有显著的抑制作用。提示日本鬼鲉蜇伤造成局部症状及其伴随的恶心、呕吐、心慌、胸闷等全身症状和继发感染可能与P物质和生长抑素含量变化有关。 |
| 关键词: 日本鬼鲉 粗毒 P物质 生长抑素 淋巴细胞 透明质酸酶 |
| DOI：10.11693/hyhz200004005005 |
| 分类号: |
| 基金项目:国家自然科学基金资助项目，39270645号 |
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| EFFECT OF INJURY STUNG BY STONEFISH (INIMICUS JAPONICUS) ON THE CONTENTS OF SUBSTANCE P AND SOMATOSTATIN AND ACTIVITY OF LYMPHOCYTES IN RATS |
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ZHANG Ke-ling, LIU Xiao-ping, DU Wei, YU Ye-jun, SUN Yan-zeng, WANG Hai-qing, QIN Shi-de
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Medical College of Qingdao University, Qingdao266021
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| Abstract: |
| The injury stung by stonefish Inimicus japonicus is a professional disease for fishermen, which cause intense pain, swelling, severe infection around the wound, accompanying some systemic symptoms. In order to identify the mechanism of the damage, the contents of SP and SS in the wounded tissue, plasma, spinal cord (L1–5), medulla oblangate, midbrain, hypothalamus and immune organs of rats and the activity of lymphocytes were detected. The activity of hyaluronidase in crude venom was determined. Compared to that in control group rats, the content of SP in the rats with injection of crude venom increases in muscle, medullary bulb, midbrain, thymus and spleen, but decreases in hypothalamus. The content of SS is enhanced in spinal cord, medullary bulb, midbrain and thymus. The crude venom could inhibit the activities of lymphocytes in vitro and in vivo. The results suggest that the local symptoms caused by Inimicus japonicus may be mediated by SP and the systemic signs may be related to the changes of SP and SS. |
| Key words: Stonefish (Inimicus japonicus), Crude venom, Substance P (SP), Somatostatin (SS), Lymphocyte, Hyaluronidase |
