| 摘要: |
| 于1997年下半年在大连石庙海区采集石莼、孔石莼、砺菜、缘管浒苔、肠浒苔、管浒苔、刚毛藻等7种海藻样品，采用RAPD技术对大连地区绿藻门石莼属和浒苔属进行了初步的分子系统学研究。所试60个引物中有32个经扩增得到了237个多态片段。应用PHYLIP软件包，按照UPGMA法和N-J法聚类分析的结果均表明，缘管浒苔(Enteromorpha linza)与石莼属有较近的亲缘关系，应深入探究其分类地位。 |
| 关键词: 石莼属 浒苔属 RAPD |
| DOI：10.11693/hyhz200004011011 |
| 分类号: |
| 基金项目:辽宁省优秀青年科研人才培养基金资助项目，963005号 |
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| APPLICATION OF RAPD IN ULVA AND ENTEROMORPHA (CHLOROPHYTA) |
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YANG Jun1, AN Li-jia1, WANG Qian1, WANG Hong-wei1, SU Qiao1, KANG Xiao-hui2
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1.Department of Bioengineering, Dalian University of Technology, Dalian116012;2.Dalian Medical College Dandong Branch, Dandong118002
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| Abstract: |
| To study the classification of Ulva and Enteromorpha at molecular level, genetic variation in Ulva lactuca, U. pertusa, U. conglobata, Enteromorpha linza, E. intestinalis, E. tubulosa and Cladophora sp., (obtained from Shi Miao, Dalian in 1997), was detected by using Random Amplified Polymorphic DNA (RAPD) markers. Amplifications with 32 primers under predetermined optional conditions (samples were first at 96°C for 10 minutes and followed by 45 cycles of 60 seconds at 94°C, 80 seconds at 36°C and 140 seconds at 72°C, and then held at 72°C for 10 minutes) generated a total of 237 prominent amplification products. The amplified fragments were scored as present (1) or absent (0) for each sample and the similarity value (S) was calculated by using Nei and Li’s matching coefficient method. The value of D (D=1–S) was used to evaluate genetic distances between species. The dendrograms were built with methods of UPGMA and Neighbor-joining on the basis of genetic distances by using PHYLIP software. The results from the two cluster analysis methods were similar in general. And Enteromorpha linza was first clustered with Ulva, not with Enteromorpha, which was shown by the two phylogenetic trees. It suggests that random amplified polymorphic DNA (RAPD) markers is useful in discriminating Ulva and Enteromorpha, and that the relationship between E. linza and Ulva is much close. |
| Key words: Ulva, Enteromorpha, RAPD |
