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重组酵母菌对牙鲆非特异性免疫能力的影响
王宏田, 张培军
中国科学院海洋研究所实验海洋生物学开放研究实验室青岛266071

摘要:

于1999年10—11月间在荣成市寻山养殖场实验了饲喂含有大麻哈鱼生长激素基因的重组酵母对1龄牙鲆非特异性免疫能力的影响。实验分为对照组和3个实验组。对照组的饵料中不含重组酵母菌,实验组的饵料中分别含有重组酵母菌0.25‰、0.50‰、0.75‰(W/W)。实验表明,通过投喂重组酵母菌,可以增加牙鲆血清蛋白的含量,增强牙鲆血清中溶菌酶及抗蛋白酶物质的活性,这种影响与重组酵母菌的含量成正相关。

关键词: 牙鲆重组酵母菌非特异性免疫

DOI：10.11693/hyhz200006007007

分类号:

基金项目:国家“973”课题资助项目，G1999012003号

EFFECTS OF RECOMBINANT YEAST ON THE NON-SPECIFIC IMMUNE ACTIVITIES OF PARALICHTHYS OLIVACEUS

WANG Hong tian, ZHANG Pei jun

Experimental Marine Biology Laboratory,Institute of Oceanology, The Chinese Academy of Sciences,Qingdao,266071

Abstract:

Experiments were conducted at Rongcheng Xunshan Fishery to analyze the effects of recombinant yeast on the immune parameters of flounder, Paralichthys olivaceus. No recombinant yeast was fed in the control group. In the experimental groups, Groups I, Groups II, Groups III, 0.25‰, 0.50‰, 0.75‰ (W/W) recombinant yeast was mixed in the diet, respectively, to feed the fish. The results show that, in the control group, Groups I, Groups II and Groups III, the total serum protein contents were 18.9–25.6mg/ml, respectively; and the lysozyme activities were 2.93–5.38 unit/(min*mg); the antiprotease activities were 2.72–5.07×10^-2 unit/(min*mg), respectively. Hence, when recombinant yeast was mixed with the diet, some Non-specific immune parameters of Paralichthys olivaceus were enhanced.

Key words: Paralichthys olivaceus, Recombinant yeast, Non-specific immune activities

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